Single cell transcriptional analysis reveals novel innate immune cell types [PDF]
PeerJ, 2014 Single-cell analysis has the potential to provide us with a host of new knowledge about biological systems, but it comes with the challenge of correctly interpreting the biological information.
Linda E. Kippner +3 more
doaj +6 more sources
Discovery of alternative polyadenylation dynamics from single cell types [PDF]
Computational and Structural Biotechnology Journal, 2020 Alternative polyadenylation (APA) occurs in the process of mRNA maturation by adding a poly(A) tail at different locations, resulting increased diversity of mRNA isoforms and contributing to the complexity of gene regulatory network.
Congting Ye +2 more
doaj +2 more sources
Single-nucleus and single-cell transcriptomes compared in matched cortical cell types.
PLoS ONE, 2018 Transcriptomic profiling of complex tissues by single-nucleus RNA-sequencing (snRNA-seq) affords some advantages over single-cell RNA-sequencing (scRNA-seq). snRNA-seq provides less biased cellular coverage, does not appear to suffer cell isolation-based
Trygve E Bakken +27 more
doaj +4 more sources
Molecular phenotyping of plant single cell-types enhances forward genetic analysis [PDF]
Frontiers in Plant Science, 2015 Recent advances in the isolation of single cell-types in plants provides an opportunity to conduct detailed analyses of their molecular characteristics at high resolution.
John eSchiefelbein
doaj +2 more sources
High-Throughput Single-Cell Transcriptome Profiling of Plant Cell Types [PDF]
Cell Reports, 2019 Summary: Single-cell transcriptome profiling of heterogeneous tissues can provide high-resolution windows into developmental dynamics and environmental responses, but its application to plants has been limited.
Christine N. Shulse +10 more
doaj +5 more sources
Identifying cell types from spatially referenced single-cell expression datasets.
PLoS Computational Biology, 2014 Complex tissues, such as the brain, are composed of multiple different cell types, each of which have distinct and important roles, for example in neural function.
Jean-Baptiste Pettit +5 more
doaj +4 more sources
Cell type identification from single-cell transcriptomes in melanoma [PDF]
BMC Medical Genomics, 2021 Background Single-cell sequencing approaches allow gene expression to be measured at the single-cell level, providing opportunities and challenges to study the aetiology of complex diseases, including cancer.
Qiuyan Huo +5 more
doaj +3 more sources
Toward Plant Single Cell Type Systems Biology [PDF]
Frontiers in Plant Science, 2016 The molecular responses of a plant to a stress and the molecular profiles of plant organs during their development and differentiation are the reflection of the contribution from different cell types composing the plant and the organs. Hence, a major limitation to understanding plant cellular and molecular responses in different cells is the ...
Marc eLibault, Sixue eChen
doaj +3 more sources
Neuronal cell types in the fly: single-cell anatomy meets single-cell genomics [PDF]
Current Opinion in Neurobiology, 2019 At around 150 000 neurons, the adult Drosophila melanogaster central nervous system is one of the largest species, for which a complete cellular catalogue is imminent. While numerically much simpler than mammalian brains, its complexity is still difficult to parse without grouping neurons into consistent types, which can number 1-1000 cells per ...
Bates, Alexander Shakeel +3 more
openaire +4 more sources
Whole-organism eQTL mapping at cellular resolution with single-cell sequencing
eLife, 2021 Genetic regulation of gene expression underlies variation in disease risk and other complex traits. The effect of expression quantitative trait loci (eQTLs) varies across cell types; however, the complexity of mammalian tissues makes studying cell-type ...
Eyal Ben-David +5 more
doaj +1 more source