Results 11 to 20 of about 63,316 (241)

Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence [PDF]

Light: Science & Applications, 2023
Conventional widefield excitation and temporal phase-shift fluorescence interference in a basic 4π-interferometer enable video rate, multiple particle tracking with isotropic, 3D precision over large volumes in cells and deep in soft materials.
Leanne Maurice, Alberto Bilenca
doaj   +2 more sources

4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector [PDF]

Nature Communications
Single-particle tracking techniques enable investigation of the complex functions and interactions of individual particles in biological environments. Many such techniques exist, each demonstrating trade-offs between spatiotemporal resolution, spatial ...
Andrea Bucci, Giorgio Tortarolo, Marcus Oliver Held, Luca Bega, Eleonora Perego, Francesco Castagnetti, Irene Bozzoni, Eli Slenders, Giuseppe Vicidomini   +8 more
doaj   +2 more sources

Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells

Molecules, 2019
Single molecule fluorescence spectroscopy has been largely implemented using methods which require tethering of molecules to a substrate in order to make high temporal resolution measurements.
Shangguo Hou, Courtney Johnson, Kevin Welsher   +2 more
doaj   +3 more sources

Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single-Particle Tracking Microscopy

Entropy, 2021
In this work, we present a 3D single-particle tracking system that can apply tailored sampling patterns to selectively extract photons that yield the most information for particle localization.
Chen Zhang, Kevin Welsher
doaj   +3 more sources

FidlTrack: high-fidelity structure-aware single particle tracking resolves intracellular molecular motion in organelles sensing APP processing [PDF]

Nature Communications
Single Particle Tracking (SPT) is a powerful technique for elucidating the dynamic behaviours of macromolecules within live cells. However, SPT’s application to subcellular environments is hampered by the error-proneness of tracking at high particle ...
Pierre Parutto, Yutong Yuan, Valentina Davì, Roger Pons-Lanau, Svenja Ebeling, Karnika Gupta, Francesca Bottanelli, Maria F. Garcia-Parajo, Felix Campelo, Clemens F. Kaminski, Joseph E. Chambers, Jonathon Nixon-Abell, Edward Avezov   +12 more
doaj   +2 more sources

Fast Single-Particle Tracking of Membrane Proteins Combined with Super-Resolution Imaging of Actin Nanodomains [PDF]

Scientific Data
Membrane protein dynamics regulates cell functions by initiating downstream signaling cascades. The cell membrane is compartmentalized into nanodomains by actin structures, restricting lateral protein diffusion.
Hanieh Mazloom-Farsibaf, William K. Kanagy, Diane S. Lidke, Keith A. Lidke   +3 more
doaj   +2 more sources

Real-Time Feedback-Driven Single-Particle Tracking: A Survey and Perspective. [PDF]

Small, 2022
van Heerden B, Vickers NA, Krüger TPJ, Andersson SB.   +3 more
europepmc   +2 more sources

Particle-by-Particle In Situ Characterization of the Protein Corona via Real-Time 3D Single-Particle-Tracking Spectroscopy*. [PDF]

Angew Chem Int Ed Engl, 2021
Tan X, Welsher K.
europepmc   +2 more sources

Intracellular transport dynamics revealed by single-particle tracking. [PDF]

Biophys Rep, 2021
Zhang ML, Ti HY, Wang PY, Li H.
europepmc   +2 more sources

Multi-color single particle tracking with quantum dots. [PDF]

PLoS ONE, 2012
Quantum dots (QDs) have long promised to revolutionize fluorescence detection to include even applications requiring simultaneous multi-species detection at single molecule sensitivity. Despite the early promise, the unique optical properties of QDs have
Eva C Arnspang, Jonathan R Brewer, B Christoffer Lagerholm   +2 more
doaj   +1 more source