Results 171 to 180 of about 758 (215)
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THE MECHANISM OF CONSERVATIVE SITE-SPECIFIC RECOMBINATION
Annual Review of Genetics, 1988Cet article de synthese decrit brievement les roles biologiques varies de la recombinaison site-specifique conservative (RSSC) et decrit plus particulierement les proteines et les composantes de l'ADN des systemes RSSC et les mecanismes de ...
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Site-specific recombination by Tn3 resolvase
Trends in Genetics, 1989Site-specific recombination processes in microbes bring about precise DNA rearrangements which have diverse and important biological functions. The sites and recombinase enzymes used for these processes fall into two distinct families. Here we describe how experiments with one family, exemplified by the resolution system of transposon Tn3, have ...
W M, Stark, M R, Boocock, D J, Sherratt
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Site specific radioiodination of recombinant hirudin
Analytical Biochemistry, 1990Recombinant hirudin variant rHV2-Lys47 was radioiodinated using the chloramine-T method. Depending on the reaction pH, the two tyrosine residues, Tyr3 and Tyr63, responded differently to iodination but without change in total iodination yield. Of the incorporated -125 iodine 80% was located on Tyr3 at pH 7.4, but 65% was found on Tyr63 at pH 4.
A, Tuong +3 more
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Site-specific recombination in Zea mays
Theoretical and Applied Genetics, 2005The elimination of marker genes after selection is recommended for the commercial use of genetically modified plants. We compared the applicability of the two site-specific recombination systems Cre/lox and Flp/FRT for marker gene elimination in maize plants.
S, Kerbach, H, Lörz, D, Becker
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Site-specific recombination in bacteriophage lambda
Journal of Molecular Biology, 1977Abstract Lambda attB-attP is a derivative of bacteriophage lambda that contains both attB and attP, two sites required for integrative recombination. Lambda attB-attP can undergo int-mediated recombination to yield progeny phages whose DNA is 15% shorter than that of the parental phage.
J, Engler, R B, Inman
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Inducible site‐specific recombination in myelinating cells
genesis, 2002AbstractSummary: To explore the function of genes expressed by myelinating cells we have developed a model system that allows for the inducible ablation of predetermined genes in oligodendrocytes and Schwann cells. The Cre/loxP recombination system provides the opportunity to generate tissue‐specific somatic mutations in mice.
Doerflinger, Nathalie +2 more
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2000
Site-specific recombination occurs in the temperate response of bacteriophages, the integration of plasmids, and the movement of transposons. While there are elements of the basic recombination pathways discussed in Chapter 5, there are unique aspects to each of these processes.
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Site-specific recombination occurs in the temperate response of bacteriophages, the integration of plasmids, and the movement of transposons. While there are elements of the basic recombination pathways discussed in Chapter 5, there are unique aspects to each of these processes.
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Introduction to site-specific recombination
2005Recombination provides a means for creating genetic variety. Exchange of information within gene pools expands their diversity and enhances the choices available for natural selection to act on. Recombination can be broadly divided into two classes: the highly pervasive “homologous” recombination and the more specialized “site-specific recombination.”
Makkuni Jayaram, Ian Grainge
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Determinants of directionality in lambda site-specific recombination
Cell, 1984The DNA structural features governing directionality in lambda site-specific recombination are shown to reside in regions of the phage attachment site more than 70 bp to the left and more than 40 bp to the right of the cross-over region. Disposition of these sequences on the same attachment site in integration, and on different attachment sites in ...
W, Bushman, S, Yin, L L, Thio, A, Landy
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Site-specific recombination: developments and applications
Current Opinion in Biotechnology, 1994Site-specific DNA recombination systems have considerable utility in manipulating DNA and can facilitate many cloning and gene transfer techniques. The ability of a number of recombinases to catalyze efficient DNA recombination in higher eukaryotes has important and exciting consequences for precise chromosome and transgene engineering. Exploitation of
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