Results 221 to 230 of about 5,811,187 (302)

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

An In Silico and In Vitro Approach Identified Potential Trypanothione Synthetase Inhibitors with Trypanocidal Activity. [PDF]

Molecules
Gómez-Escobedo R, Méndez-Álvarez D, Paz-González AD, Ortiz-Pérez E, Vázquez-Jiménez LK, Martínez-Vázquez AV, Delgado-Maldonado T, Quintero-Solano JM, Vázquez C, Saavedra E, Avalos-Navarro G, Vázquez K, Rivera G, Nogueda-Torres B.   +13 more
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Structural basis of menaquinone reduction by succinate dehydrogenase from Chloroflexus aurantiacus. [PDF]

Nat Commun
Zhang X, Wu J, Wang J, He H, Liu A, Hong X, Yu Y, Pei X, Fang X, Xin Y, Yu L, Tian C, Xu X.   +12 more
europepmc   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

A Single Flavoenzyme Forges the Pentacyclic Skeleton of α-Cyclopiazonic Acid. [PDF]

J Am Chem Soc
Wang M, Cheng T, Zhang W, Kuo YM, Li Y, Yan Y, Hisao PY, Chang CY, Wang N.   +8 more
europepmc   +1 more source

Impact of the three-fold channel substitution D131N on kinetics of translocation of Fe²⁺ across the protein coat is more severe for human cytosolic H-chain ferritin than for human mitochondrial ferritin.

Dalton Trans
Bugg Z, Hazlewood C, Hemmings AM, Bradley JM, Le Brun NE.   +4 more
europepmc   +1 more source

Enabling the synthesis of multi-payload thio-antibody conjugates through the use of pyridazinediones, <i>p</i>-anisidine derivatives and various click chemistries. [PDF]

RSC Chem Biol
McMahon C, Queval CJ, Thanasi IA, Lau DHW, Howell M, Wang N, Lee MTW, Gaynord JS, Baker JR, Chudasama V.   +9 more
europepmc   +1 more source

Lid loop-mediated proton transfer revealed in the Fe/αKG-dependent decarboxylase TraH. [PDF]

Commun Chem
Zheng X, Ge R, Guo Z, Girbig M, Freitag J, Li A, Hochberg GKA, Li SM, Bange G, Zheng L.   +9 more
europepmc   +1 more source

Structural Insights Into CO₂ Transport Pathways in a W-Formate Dehydrogenase: Structural Basis for CO₂ Reduction. [PDF]

Angew Chem Int Ed Engl
Vilela-Alves G, Manuel RR, Martins G, Carpentier P, Raczyńska A, Szaleniec M, Pereira IAC, Romão MJ, Mota C.   +8 more
europepmc   +1 more source