Results 231 to 240 of about 4,417,534 (309)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Neonicotinoid and s-triazine pesticide transport dynamics in a small karst agricultural watershed. [PDF]

J Environ Qual
Kibuye HJ, Veith TL, Groh TA, Preisendanz HE.   +3 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Progress in Shortening Treatment Courses for Bone Metastases in a Statewide Quality Consortium. [PDF]

Adv Radiat Oncol
Higgins LM, Yin HM, Griffith K, Johnson-Olokesusi J, Paradis KC, Bhatt AK, Critchfield L, Baldwin K, Narayana V, Messiha H, Davis J, Fakhreddine M, Abu-Isa E, Hayman JA, Michigan Radiation Oncology Quality Consortium as part of the Blue Cross Blue Shield of Michigan and Blue Care Network of Michigan Value Partnerships Program.   +14 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Patient Satisfaction Using Guselkumab Self-administered Using the One-Press Device for Treatment of Moderate-to-Severe Psoriasis: Results from a National, Prospective, Real-World Study in Portugal (CERES Study). [PDF]

Dermatol Ther (Heidelb)
Torres T, Sousa JT, Antunes J, Mendes-Bastos P, Brasileiro A, Neto V, Henrique M, Pimenta R, Magina S, Lourenço D, Mota F.   +10 more
europepmc   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

SynPoC: a high-quality generative diffusion model for transforming ultra-low-field point-of-care MRI using high-field MRI representations. [PDF]

Sci Rep
Islam KT, Dayarathna S, Zhong S, Zakavi P, Kavnoudias H, Farquharson S, Durbridge G, Sun H, Bacchi S, Egan GF, Barth M, Dwyer A, McMahon KL, Parizel PM, Law M, Chen Z.   +15 more
europepmc   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Evaluation of a trauma center-based, technology enhanced stepped care intervention to promote the mental health recovery of adolescent traumatic injury survivors. [PDF]

Contemp Clin Trials Commun
Davidson TM, Skojec T, Li P, Ridings LE, Espeleta H, German S, Mueller M, Gates R, Mooney D, Russell R, deRoon-Cassini T, Ruggiero K.   +11 more
europepmc   +1 more source