Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Effects of fasting on HDL particle function and size distribution. [PDF]
Agus JK, Zivkovic AM.
europepmc +1 more source
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
Impact of the Nano-Precipitation Size Distribution on the Mechanical Behavior of Nickel-Based Alloys by Experiment and Simulation. [PDF]
Wen Y +10 more
europepmc +1 more source
Digital twins to accelerate target identification and drug development for immune‐mediated disorders
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley +1 more source
Focus on the role of mixed micelles and lipid droplets in the oxidative stability of oil-in-water emulsions using size distribution Taylor dispersion analysis. [PDF]
Durand E +4 more
europepmc +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Hybrid Steel Fiber Design in Ultra-High-Performance Concrete Containing Coarse Aggregate Using Pore Size Distribution Within Coarse Aggregate Skeleton. [PDF]
Tang R, Du Y, Zhang J, Kong L.
europepmc +1 more source
Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source
Geographical characteristics of raindrop size distribution for rainy season in Eastern China. [PDF]
Wang H, Wang J, Ren G, Wang W, Zhang D.
europepmc +1 more source

