Results 1 to 10 of about 59,567 (351)

Spliceosomal snRNA Epitranscriptomics [PDF]

Frontiers in Genetics, 2021
Small nuclear RNAs (snRNAs) are critical components of the spliceosome that catalyze the splicing of pre-mRNA. snRNAs are each complexed with many proteins to form RNA-protein complexes, termed as small nuclear ribonucleoproteins (snRNPs), in the cell ...
Pedro Morais, Hironori Adachi, Yi-Tao Yu
doaj   +4 more sources

Structures and mechanisms of U6 snRNA m6A modification by METTL16 [PDF]

Nature Communications
The N 6-methyladenosine (m6A) modification in U6 snRNA, catalyzed by METTL16 using S-adenosylmethionine (SAM) as the methyl donor, is required for efficient and accurate pre-mRNA splicing.
Jue Ju, Kozo Tomita
doaj   +4 more sources

A single m6A modification in U6 snRNA diversifies exon sequence at the 5’ splice site

Nature Communications, 2021
Spliceosomal U6 snRNA is modified by m6A. Here the authors show that m6A modification on U6 snRNA promotes efficient recognition of the splice site through cooperating with U5 snRNA, indicating that U6 snRNA m6A allows the 5’ exon sequence variation.
Yuma Ishigami, Takayuki Ohira, Yui Isokawa, Yutaka Suzuki, Tsutomu Suzuki   +4 more
doaj   +2 more sources

m6A modification of U6 snRNA modulates usage of two major classes of pre-mRNA 5’ splice site

eLife, 2022
Alternative splicing of messenger RNAs is associated with the evolution of developmentally complex eukaryotes. Splicing is mediated by the spliceosome, and docking of the pre-mRNA 5’ splice site into the spliceosome active site depends upon pairing with ...
Matthew T Parker, Beth K Soanes, Jelena Kusakina, Antoine Larrieu, Katarzyna Knop, Nisha Joy, Friedrich Breidenbach, Anna V Sherwood, Geoffrey J Barton, Sebastian M Fica, Brendan H Davies, Gordon G Simpson   +11 more
doaj   +2 more sources

Regulation of expression of human RNA polymerase II-transcribed snRNA genes [PDF]

Open Biology, 2017
In addition to protein-coding genes, RNA polymerase II (pol II) transcribes numerous genes for non-coding RNAs, including the small-nuclear (sn)RNA genes.
Joana Guiro, Shona Murphy
doaj   +2 more sources

Evaluating cell-specific gene expression using single-cell and single-nuclei RNA-sequencing data from human pancreatic islets of the same donors [PDF]

Scientific Reports
Single-cell and single-nuclei RNA-sequencing (scRNA-seq and snRNA-seq) analyze cell-specific transcriptomes. However, only snRNA-seq applies to frozen biobanked samples.
Karin Engström, Åsa Nilsson, Jones K. Ofori, Nils Wierup, Karl Bacos, Charlotte Ling   +5 more
doaj   +2 more sources

snRNA 3' End Processing by a CPSF73-Containing Complex Essential for Development in Arabidopsis. [PDF]

PLoS Biology, 2016
Uridine-rich small nuclear RNAs (snRNAs) are the basal components of the spliceosome and play essential roles in splicing. The biogenesis of the majority of snRNAs involves 3' end endonucleolytic cleavage of the nascent transcript from the elongating DNA-
Yunfeng Liu, Shengjun Li, Yuan Chen, Athen N Kimberlin, Edgar B Cahoon, Bin Yu   +5 more
doaj   +2 more sources

U6 snRNA expression prevents toxicity in TDP-43-knockdown cells. [PDF]

PLoS ONE, 2017
Depletion of amyotrophic lateral sclerosis (ALS)-associated transactivation response (TAR) RNA/DNA-binding protein 43 kDa (TDP-43) alters splicing efficiency of multiple transcripts and results in neuronal cell death.
Masao Yahara, Akira Kitamura, Masataka Kinjo   +2 more
doaj   +2 more sources

Mutational Analysis Reveals Functional Roles of METTL16 Domains and Residues [PDF]

Biology
Human methyltransferase-like protein 16 (METTL16) installs N6-methyladenosine on U6 small nuclear RNA (snRNA) and other RNAs. Multiple X-ray crystal structures of METTL16 have been published; however, we do not yet fully understand the structure–function
Kurtis Breger, Ian P. Schowe, Noah A. Springer, Nathan J. O’Leary, Agnieszka Ruszkowska, Carlos Resende, Jessica A. Brown   +6 more
doaj   +2 more sources

N 2-methylguanosine modifications on human tRNAs and snRNA U6 are important for cell proliferation, protein translation and pre-mRNA splicing

Nucleic Acids Research, 2023
Modified nucleotides in non-coding RNAs, such as tRNAs and snRNAs, represent an important layer of gene expression regulation through their ability to fine-tune mRNA maturation and translation.
Can Wang, Nathalie Ulryck, Lydia Herzel, Nicolas Pythoud, Nicole Kleiber, V. Guérineau, Vincent Jactel, Chloé Moritz, M. Bohnsack, C. Carapito, D. Touboul, K. Bohnsack, M. Graille   +12 more
semanticscholar   +1 more source