Decoding adipocyte heterogeneity through single-nucleus transcriptomics unveils subtype-specific adipocytes orchestrate immunosuppressive niches in breast cancer. [PDF]
Tong Y +8 more
europepmc +1 more source
Versatile and Selective Biomolecule Pulldown with Combinatorial DNA‐Crosslinked Polymers
We present a programmable smart polymer system for universal, sequence‐selective bioseparation under native conditions. Polymer phase separation is achieved through combinatorial DNA crosslinking, enabling the capture of DNA, RNA, or proteins in a homogeneous solution with high pulldown efficiency and near‐zero background binding.
Sarah K. Speed +3 more
wiley +1 more source
Hepatic adaptation to chronic metabolic stress primes tumorigenesis. [PDF]
Tzouanas CN +33 more
europepmc +1 more source
RNA‐Lebendzellmikroskopie via click‐funktionalisierter TriPPPro Nucleotid‐Reporter
Die intrazelluläre Zufuhr von trans‐Cycloocten und Bicyclo[6.1.0]nonin als sterisch anspruchsvolle Gruppen für die Diels–Alder‐Reaktion mit inversem Elektronenbedarf wurde durch die Triphosphat‐Prodrug‐Strategie (TriPPPro) erreicht. Nach der Hydrolyse innerhalb der Zelle werden die modifizierten Uridine und Cytidine metabolisch in die neu entstehende ...
J. Iven H. Knaack +6 more
wiley +1 more source
Single-cell nuclear RNA-sequencing reveals dynamic changes in breast muscle cells during the embryonic development of Ding'an goose. [PDF]
Xu T +8 more
europepmc +1 more source
Live‐Cell RNA Imaging via Clickable TriPPPro Nucleotide Reporters
The intracellular delivery of trans‐cyclooctene and bicyclo[6.1.0]nonyne as sterically demanding groups for the inverse electron‐demand Diels–Alder was achieved by the triphosphate prodrug (TriPPPro) strategy. Once hydrolyzed inside cells, the modified uridines and cytidines are metabolically incorporated into nascent RNA, are labelled by a dual ...
J. Iven H. Knaack +6 more
wiley +1 more source
A streamlined and comprehensive protocol for the generation and multi-omic analysis of human monocyte-derived macrophages. [PDF]
Palmer OG +4 more
europepmc +1 more source

