Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Social Acceptance of Mobile Health among Young Adults in Japan: An Extension of the UTAUT Model. [PDF]
Int J Environ Res Public Health, 2022 Cao J +4 more
europepmc +1 more source
FEBS Open Bio, EarlyView.This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
Social acceptance of nursing during the coronavirus pandemic: COVID-19 an opportunity to reform the public image of nursing. [PDF]
Nurs Open, 2022 Zamanzadeh V +2 more
europepmc +1 more source
Social acceptance research guide
, 2023 This report seeks to provide a reflection on the research completed within the network by combining the accumulated knowledge of the MISTRAL network on key social acceptance variables and key papers addressing them, providing a ‘travel guide’ to social acceptance research for those embarking on research on social dimensions of renewable energy and the ...
openaire +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Social acceptance of livestock-administered endectocides for malaria control in Vhembe District, Limpopo Province, South Africa. [PDF]
Malar J, 2022 Makhanthisa TI +3 more
europepmc +1 more source
Education as Liberation? [PDF]
Scholars have long speculated about education’s political impacts, variously arguing that it promotes modern or pro-democratic attitudes; that it instills acceptance of existing authority; and that it empowers the disadvantaged to challenge authority. To
Edward Miguel +3 more
core
Digital twins to accelerate target identification and drug development for immune‐mediated disorders
FEBS Open Bio, EarlyView.Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley +1 more source
Multi-Criteria Decision Analysis for Assessing Social Acceptance of Strategies to Reduce Antimicrobial Use in the French Dairy Industry. [PDF]
Antibiotics (Basel), 2022 Manriquez D +4 more
europepmc +1 more source