Results 251 to 260 of about 2,297,329 (297)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Technology, Complexity, and Social Decision

, 2019
openaire   +1 more source

Food culture as a mechanism of social bonding and social identity in primates. [PDF]

Front Hum Neurosci
Roberts AI, Roberts SGB.
europepmc   +1 more source

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The Complexity of Social Complexity: A Quantitative Multidimensional Approach for Studies of Social Organization

American Naturalist, 2020
AbstractThe rapid increase in "big data" during the postgenomic era makes it crucial to appropriately measure the level of social complexity in comparative studies. We argue that commonly used qualitative classifications lump together species showing a broad range of social complexity and falsely imply that social evolution always progresses along a ...
Jacob G Holland, Guy Bloch
exaly   +3 more sources

Bioarchaeology and Social Complexity:

2017
The closing section of the volume contains commentary and considerations on the bioarchaeology of social complexity. The authors identify several broad themes and issues crosscutting the chapters and offer constructive critiques for future bioarchaeological studies of hierarchy, heterarchy, and other expressions of social organization in antiquity ...
Klaus, Haagen D., Cohen, Mark N., Danforth, Marie Elaine, Harvey, Amanda R.   +3 more
openaire   +1 more source

Cognitive Complexity and Sociality

British Journal of Social and Clinical Psychology, 1969
A discussion of Bieri's definition of ‘cognitive complexity’ within the general framework of Personal Construct Theory, and specifically in terms of the theoretical implications of Kelly's Sociality Corollary , led to the hypothesis that relatively cognitively complex persons infer the ...
openaire   +2 more sources

Complexity Theories, Social Theory, and the Question of Social Complexity

Philosophy of the Social Sciences, 2001
In this article, the author argues that complexity theories have limited use in the study of society, and that social processes are too complex and particular to be rigorously modeled in complexity terms. Theories of social complexity are shown to be inadequately developed, and typical weaknesses in the literature on social complexity are discussed ...
openaire   +1 more source