Results 241 to 250 of about 14,971,537 (311)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Editorial: Non-biomedical perspectives on pain and its prevention and management - volume II. [PDF]

open access: yesFront Pain Res (Lausanne)
Johnson MI   +3 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Why and how the humanities matter for sustainability transformations: a humanities-sustainability science bridging perspective. [PDF]

open access: yesHumanit Soc Sci Commun
Horcea-Milcu AI   +6 more
europepmc   +1 more source

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