Results 131 to 140 of about 13,493,822 (297)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Cultivating Europe: Democratic Europe - Social Europe - Educated Europe [PDF]
Proceedings of the Conference "Impact of Social Sciences and Humanities for a European Research Agenda - Valuation of SSH in mission-oriented research" VIENNA 2018, 2019 Katharina Berghöfer +6 more
openaire +1 more source
FEBS Open Bio, EarlyView.The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley +1 more source
Science Europe Multi-annual Action Plan 2021-2026 [PDF]
Ce plan d’action pluriannuel propose une série d’actions-cadres (Section 3) pour guider la mise en œuvre de la stratégie de Science Europe pour planifier 2021-2026 conformément à la capacité, à la vision, à la mission. Le plan d’action pluriannuel décrit
Science Europe
core
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins
FEBS Open Bio, EarlyView.Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley +1 more source
The economic burden of anesthetic-related adverse events: a systematic review and empirical analysis
BMC AnesthesiologyBackground While extensive evidence is available on the adverse events (AEs) related to agents used to perform general anesthesia and procedural sedation from a clinical standpoint, much less is known about their economic impact on healthcare systems ...
Patrizio Armeni +5 more
doaj +1 more source
Reinventing Social Solidarity Across Europe
, 2011 As Europe's public realms face upheaval, this is the first book to identify how social solidarity is being re-invented from below and redefined from above.
Ellison, Marion
core
FEBS Open Bio, EarlyView.NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks +4 more
wiley +1 more source