Results 121 to 130 of about 12,087,707 (288)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Overview of MediaEval 2011 rich speech retrieval task and genre tagging task [PDF]

, 2011
The MediaEval 2011 Rich Speech Retrieval Tasks and Genre Tagging Tasks are two new tasks oered in MediaEval 2011 that are designed to explore the development of techniques for semi-professional user generated content (SPUG).
Eskevich, Maria, Kofler, Christoph, Schmiedeke, Sebastian, Ordelman, Roeland, Larson, Martha, Jones, Gareth J.F.   +5 more
core   +2 more sources

A corpus-based real-time text classification and tagging approach for social data

Frontiers in Computer Science
With the rapid accumulation of large amounts of user-generated content through social media, social data reuse and integration have gained increasing attention recently.
Atia Bano Memon, Atia Bano Memon, Dileep Kumar Sootahar, Kirshan Kumar Luhana, Kyrill Meyer, Kyrill Meyer   +5 more
doaj   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Elements of social representation theory incollaborative tagging systems

Transinformação
This article discusses the information representation process based on the Moscovici's Social Representation Theory and domain analysis in Information Science.
Patricia Zeni Marchiori, Andre Luiz Appel, Eduardo Michellotti Bettoni, Denise Fukumi Tsunoda, Frank Coelho de Alcântara   +4 more
doaj  

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source

The C‐terminal truncated splicing variant of NK1R negatively modulates substance P‐stimulated NK1R signaling

FEBS Open Bio, EarlyView.
The neurokinin 1 receptor exists as full‐length (NK1L) and C‐terminally truncated (NK1S) splice variants. We show that NK1S heterodimerizes with NK1L, impairing Gαq coupling and Ca2+ mobilization while enhancing β‐arrestin1 recruitment. NK1S suppresses substance P‐driven gene expression and cell migration, revealing NK1S as an endogenous biased ...
Lan Phuong Nguyen, Duc Trung Nguyen, Minyoung Cho, Jihun Kim, Soyeon In, Thai Uy Nguyen, Sunghoon Hurh, Beom Jin Park, Jong‐Ik Hwang   +8 more
wiley   +1 more source

Introducción a las folksonomías: Definición, características y diferencias con los modelos tradicionales de indización = Introduction to folksonomies: Definition, Characteristics and Differences with Tradicional Indexing Models

Información, Cultura y Sociedad, 2013
El artículo repasa los hallazgos y opiniones de teóricos e investigadores que se han dedicado a analizar el fenómeno de las folksonomías. Se rescatan las principales definiciones del concepto de folksonomía, sus características, los tipos de folksonomías
Nadina Yedid
doaj  

Research Perspectives on Social Tagging [PDF]

, 2008
Social tagging has emerged as one of the most popular social software tools available online. Originating from Del.icio.us, social tagging capabilities can now be found on a number of major music, news, video, and commercial websites, as well as on ...
Nguyen, Lilly Uyen, Zollers, Alla, Moore, Tony, Russell, Terrell, Stutzman, Fred   +4 more
core