Results 181 to 190 of about 6,322,298 (304)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Adaptive evolution resulted in three subtypes of the <i>Riemerella anatipestifer crpR1</i> gene. [PDF]

J Bacteriol
Wang J, Du X, Yin H, Zhang X, Wang S, Liao H, Zhang X, Zheng Y, Hu Q.   +8 more
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Prognostic Value of Spatial and Topological Features of Tumor Microenvironment in Classic Hodgkin Lymphoma. [PDF]

Oncol Res
Bernal-Florindo I, Raposo-Puglia JA, Ruiz FA, Perez-Requena J, Benavides-de la Fuente C, Galan J, Berruezo-Salazar MJ, Garcia-Rojo M, Fernandez-Ponce C, Santisteban-Espejo A.   +9 more
europepmc   +1 more source

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

FEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento, Danniel Pereira‐Figueiredo, Gabriella Grossi de Lima Dias, Gabriel Ferreira dos Santos, Mariana Rodrigues Pereira, Rafael Brito, Karin C Calaza   +6 more
wiley   +1 more source

Gel-confined strain amplifies FRET efficiency toward red emission enhancement beyond pressure quenching. [PDF]

Sci Adv
Yu X, Hu H, Xie Y, Niu G, He X, Wang J, Sui L, Yuan K, Wang K, Xiao G, Wei H, Zou B.   +11 more
europepmc   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

Testing the validity and adequacy of linguistic phylogenetic analyses. [PDF]

PLoS Comput Biol
King B.
europepmc   +1 more source

Early‐life high‐fat diet exposure increases Achilles tendon stiffness and induces transcriptomic alterations

FEBS Open Bio, EarlyView.
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin, Ziyang Yuan, Wenli Jiang, Ai Guo   +3 more
wiley   +1 more source

Evolutionary Tracing and Taxonomic Implications of the Mitochondrial Genome of <i>Gephyrocharax atracaudatus</i> (Meek and Hildebrand, 1912). [PDF]

Biology (Basel)
Liu Z, Huang Y, Yang L, Ye J, Wu H, Pan J, Shan C, Shan Y, Wang W, Wang J, Feng Z, Chen S.   +11 more
europepmc   +1 more source