Results 161 to 170 of about 32,056,537 (304)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Hybrid CFD-DSMC Simulation of Ion Transport in Photoionization Mass Spectrometry: From Atmospheric Pressure to High Vacuum. [PDF]

Rapid Commun Mass Spectrom
Wen Z, Gao S, Huang J, Wang G, Tang Y, Liu C, Xu M, Gao H, Wang X, Yang J, Pan Y.   +10 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Correction: From architectural complexity to minimalist optimization: refining microscopic tea disease detection via Opti-YOLOv11n. [PDF]

Front Plant Sci
Hu C, Liu Z, Li Q, Yan Y, Liu F, He Y, Li X, Chen H, Yang C, Li J, Wei M, Liang Y, Yuan X.   +12 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

VicMAG, an open-source tool for visualizing circular metagenome-assembled genomes highlighting bacterial virulence and antimicrobial resistance. [PDF]

NAR Genom Bioinform
Tsuda Y, Tanizawa Y, Vu TMH, Nishimura Y, Shintani M, Abe H, Hasebe F, Kasuga I, Nagao M, Suzuki M.   +9 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

A method for detecting surface defects in hot-rolled strip steel based on deep learning

Metalurgija
Hot-rolled strip steel is a material widely used in production activities and daily life. However, the appearance of surface defects during its production process is inevitable.
H. Ren, Y. J. Zhang, J. T. Chen, X. N. Wei, H. K. Chen, P. Liu   +5 more
doaj  

BioTester: an AI-driven automated testing framework for identifying potential quality risks in bioinformatics software. [PDF]

Brief Bioinform
Lian X, Wang J, Zhu X, Dang S, Xu T.
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source