Combining melting curve analysis enhances the multiplexing capability of digital PCR. Here, we developed a 14‐plex assay to simultaneously measure single nucleotide mutations and amplifications of KRAS and GNAS, which are common driver genes in pancreatic cancer precursors. This assay accurately quantified variant allele frequencies in clinical samples
Junko Tanaka+10 more
wiley +1 more source
Ultrasound pretreatment and solvent extraction parameters effects on the nutritional characteristics of Indonesian shortfin eel (Anguilla bicolor bicolor) protein concentrate. [PDF]
Kumoro AC+6 more
europepmc +1 more source
Investigation on the Composition and Extraction Mechanism of the Soluble Species from Oily Sludge by Solvent Extraction. [PDF]
He X, Li G, Mo W, Yuan J, Wei X, Wu Y.
europepmc +1 more source
Exploration of heterogeneity and recurrence signatures in hepatocellular carcinoma
This study leveraged public datasets and integrative bioinformatic analysis to dissect malignant cell heterogeneity between relapsed and primary HCC, focusing on intercellular communication, differentiation status, metabolic activity, and transcriptomic profiles.
Wen‐Jing Wu+15 more
wiley +1 more source
Abstract We describe a case of fungal keratitis due to Beauveria bassiana in a farmer with Fuchs' dystrophy, treated with amphotericin B. Surgery with penetrating keratoplasty was necessary to resolve the lesions. Susceptibility testing and molecular sequencing permitted the identification and treatment of this rare aetiological agent of invasive ...
M. Ligozzi+8 more
wiley +1 more source
Ultrasound-Assisted Deep Eutectic Solvent Extraction of Antioxidant and Anti-Colorectal Cancer Proteins from <i>Spirulina</i> Biomass: Process Intensification, Characterization, and Bioactivity Evaluation. [PDF]
Zin MT+6 more
europepmc +1 more source
Sustainable Selective Recovery of Sulfuric Acid and Vanadium from Acidic Wastewater with Two-Step Solvent Extraction. [PDF]
Zhu X, Ma C, Li W.
europepmc +1 more source
Studies on the Liquid-Liquid Partition Systems. VI. Solvent Extraction Study of the Dissociation of Sulfurous, Selenious and Tellurous Acids in Acid Perchlorate Media [PDF]
Tatsuya Sekine+4 more
openalex +1 more source