Results 361 to 370 of about 365,020 (403)

MC1568 Enhances Histone Acetylation During Oocyte Meiosis and Improves Development of Somatic Cell Nuclear Transfer Embryos in Pig.

Cellular Reprogramming, 2018
An increasing number of studies have revealed that histone deacetylase (HDAC) mediated histone deacetylation is important for mammalian oocyte development.
Huili Wang, Wei Cui, C. Meng, Jun Zhang, Yinxia Li, Y. Qian, Guangdong Xing, Dongmin Zhao, Shaoxian Cao   +8 more
semanticscholar   +1 more source

Establishment of a Somatic Cell Bank for Indian Buffalo Breeds and Assessing the Suitability of the Cryopreserved Cells for Somatic Cell Nuclear Transfer.

Cellular Reprogramming, 2018
Biobanks of cryopreserved gametes and embryos of domestic animals have been utilized to spread desired genotypes and to conserve the animal germplasm of endangered breeds. In principle, somatic cells can be used for the same purposes, and for reviving of
N. Selokar, Papori Sharma, A. Krishna, Deepak Kumar, Dharmendra Kumar, M. Saini, Arpna Sharma, K. Vijayalakshmy, P. Yadav   +8 more
semanticscholar   +1 more source

Procedures for Somatic Cell Nuclear Transfer in the Rat

2009
Somatic cell nuclear transfer (SCNT) is a powerful tool for the investigation of the mechanisms of nuclear remodeling. In addition, SCNT may offer the possibility of introducing targeted mutations by homologous recombination in species for which ES cell technology is not available.
Yacine Cherifi, Eryao Wang, Christelle Jacquet, Alexandre Fraichard, Qi Zhou, Jean Cozzi   +5 more
openaire   +3 more sources

Animal Cloning by Somatic Cell Nuclear Transfer

2009
Animal cloning is becoming increasingly useful for its applications in biological inquiry and for its potential use in pharmaceutical, medical, and agricultural fields. Due to the complexity of the numerous steps required in reconstructing oocytes by nuclear transfer, detailed protocols are required to minimize the developmental damages inflicted ...
Jae Gyu Yoo, Lawrence C. Smith
openaire   +3 more sources

Progress in Human Somatic-Cell Nuclear Transfer

New England Journal of Medicine, 2005
The efficiency of deriving blastocysts through the nuclear transfer of embryonic stem cells has been improved by a factor of more than 14.
openaire   +3 more sources

Method for somatic cell nuclear transfer in zebrafish

2016
This chapter presents a detailed methodology for somatic cell nuclear transfer-cloning of zebrafish. We aim to place the reader in a virtual lab experience to assist acquisition of the technical skills required for reproducing the published protocol.
Jose B. Cibelli, Jose B. Cibelli, Kannika Siripattarapravat   +2 more
openaire   +3 more sources

Supplementation with spermine during in vitro maturation of porcine oocytes improves early embryonic development after parthenogenetic activation and somatic cell nuclear transfer.

Journal of Animal Science, 2016
Spermine plays an important role in protection from reactive oxygen species (ROS) in bacteria, yeast, and mammalian cells, but there are few studies on the effects of spermine on porcine oocyte maturation and subsequent embryo development.
J. Jin, Sang-Hoon Lee, Candrani Khoirinaya, A. Oh, G. A. Kim, B. C. Lee   +5 more
semanticscholar   +1 more source

Method for Somatic Cell Nuclear Transfer in Zebrafish

2011
Somatic cell nuclear transfer (SCNT) has been a well-known technique for decades and widely applied to generate identical animals, including ones with genetic alterations. The system has been demonstrated successfully in zebrafish. The elaborated requirements of SCNT, however, limit reproducibility of the established model to a few groups in zebrafish ...
Kannika, Siripattarapravat, Jose B, Cibelli   +1 more
openaire   +2 more sources

Somatic Cell Nuclear Transfer Technology

Methods in Molecular Biology, 2023

semanticscholar   +1 more source

Improvement of canine somatic cell nuclear transfer procedure

Theriogenology, 2008
The purpose of the present study on canine somatic cell nuclear transfer (SCNT) was to evaluate the effects of fusion strength, type of activation, culture media and site of transfer on developmental potential of SCNT embryos. We also examined the potential of enucleated bovine oocytes to serve as cytoplast recipients of canine somatic cells.
M. K. Kim, Jung-Eun Park, So Gun Hong, Joung Joo Kim, Yuda Heru Fibrianto, Goo Jang, Hyun Ju Oh, Mohammad Shamim Hossein, Sung Keun Kang, Byeong Chun Lee, Hyesoo Kim   +10 more
openaire   +2 more sources