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Cryopreservation of Somatic Embryos
1995Since somatic embryos (SEs) can be produced in large numbers in bioreactors (Denchev et al. 1992), and are being employed for mass micropropagation (Merkle et al. 1991; Bajaj 1991a), genetic transformation (Ellis 1993), and for the production of synthetic seed (Redenbaugh et al.
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Micropropagation Through Somatic Embryos
1995Commercial micropropagation is a challenging plant biotechnology industry that offers new methods of plant production. A micropropagation system exploits the morphogenic potential of existing growing points or meristems within the plant (Giles and Morgan 1987).
P. D. Denchev, A. I. Atanassov
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Somatic embryo production in bioreactors
Journal of Biotechnology, 1992Within the past ten years much progress has been made with various aspects of plant tissue culture and the outlook for in vitro genetic manipulation is considerably more encouraging. One of the more significant developments has been the demonstration of plant regeneration by somatic organogenesis and embryogenesis from various explants (Williams and ...
Plamen D. Denchev +2 more
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Gene Expression in Somatic Embryos
1995The developmental biology of somatic embryos is similar in many respects to that of plant zygotic embryos. For that reason, the analysis of gene expression during somatic embryogenesis has been viewed as an alternative means to investigate the genetic control of zygotic embryo development, which can be technically difficult.
H. D. Wilde, W. S. Seffens, T. L. Thomas
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Inorganic Compounds that Aid in Obtaining Somatic Embryos
2022Somatic embryogenesis (SE) is a process that allows formation of embryos from somatic cells; this biological process has different stages that first require micropropagation and conditioning of explant, and then induction, multiplication, development, and germination of somatic embryos (SoE), to obtain seedlings that will be acclimatized and grown in a
Rodrigo, Atanacio-López +6 more
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Somatic embryogenesis in soybean via somatic embryo cycling
In Vitro Cellular & Developmental Biology - Plant, 1992The objectives of the present research were: a) to develop an efficient soybean embryogenic regeneration system characterized by a high frequency of explant response and a large number of somatic embryos per explant; b) to evaluate the factors affecting somatic embryogenesis via somatic embryo cycling; and c) to identify the origin of somatic embryos ...
Wennuan Liu +2 more
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Cryopreservation of zygotic embryo axes and somatic embryos of European chestnut.
Cryo letters, 2004This work describes experiments demonstrating the feasibility of long-term conservation of Castanea sativa germplasm through cryopreservation of embryonic axes or somatic embryo clumps. Between 93% and 100% of excised embryonic axes of recalcitrant chestnut seeds survived storage in liquid nitrogen (LN) following desiccation in a laminar flow cabinet ...
Corredoira, Elena +3 more
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Immunolocalization of Proteins in Somatic Embryos
2008Plant embryogenesis requires a tight balance between cell proliferation and differentiation. In animals, embryogenesis is dependent on cell migrations, which is in contrast to plant embryogenesis where the rigid cell wall precludes migration. Therefore, plants have to position cells correctly by defining the direction of the division plane during ...
Andrei P. Smertenko, Patrick J. Hussey
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Anatomy of Somatic Embryos from Maize Embryo Cultures
Botanical Gazette, 1986Putative somatic embryos of maize (Zea mays L.) that developed directly from the scutellum of immature zygotic embryos in agar culture were investigated in thin sections and by scanning electron microscopy to confirm the mode of regeneration. After 4-6 days in culture, a row of similar-sized swellings appeared on each zygotic embryo just above the ...
J. W. McCain, T. K. Hodges
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THE UTILISATION OF AN EMBRYO BREEDER TECHNIQUE FOR MATURATION OF SOMATIC EMBRYOS
Acta Horticulturae, 1998The formation of embryos from somatic tissue may require several transfers to different media before ripe embryos can be harvested. In a semiopen system, where fresh medium is delivered continuously to the cells through a semipermeable membrane, the problem of repeated transfer can be avoided or the number of transfers reduced.
Sorvari, Seppo +3 more
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