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Somatic Embryogenesis from Immature Olive Zygotic Embryos
2022The effects of plant growth regulators (PGRs) and the explant type on the embryogenesis and plant regeneration of olive (Olea europaea L. ssp europaea var. sativa) cv. "Chetoui" were studied using immature zygotic embryos. Embryogenic callus induction was achieved on OMc medium supplemented with different concentrations of BAP and NAA at low levels ...
El Bahri, Trabelsi, Emna, Jedidi
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Somatic embryo production in bioreactors
Journal of Biotechnology, 1992Within the past ten years much progress has been made with various aspects of plant tissue culture and the outlook for in vitro genetic manipulation is considerably more encouraging. One of the more significant developments has been the demonstration of plant regeneration by somatic organogenesis and embryogenesis from various explants (Williams and ...
Plamen D. Denchev +2 more
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Somatic embryos of daylily in space
Advances in Space Research, 1999Poor growth and nuclear abnormalities observable in some space-grown plants have been hypothesized as due to a combination of factors such as degree of development, the specific way the plants are grown and the way they experience multiple stresses, some of which are space-specific.
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Static suspension culture of carrot somatic embryos
Journal of Bioscience and Bioengineering, 2005Three culture methods of carrot somatic embryos were compared: conventional shake flask culture, static suspension culture using Erlenmeyer flasks, and static suspension culture using petri dishes. Compared with the former two cultures, the last one resulted in an enhanced ratio of torpedo-shaped embryos and suppressed cotyledonary-stage embryo ...
Hongmei, Li, Kenji, Kurata
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Orchid protocorm‐like bodies are somatic embryos
American Journal of Botany, 2013• Premise of the study: Protocorm‐like bodies (PLBs) of orchids are important in orchid micropropagation and outwardly resemble somatic embryos in form and development. To determine whether PLBs are truly embryogenetic, we compared PLBs with somatic embryos and zygotic embryos to determine whether they had similar surface molecules and whether ...
Yung-I, Lee +2 more
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Plant regeneration from somatic embryos of Taxus brevifolia
Plant Cell Reports, 1996Taxusbrevifolia is the source of paclitaxel (Taxol®), an anticancer drug. A method for regeneration ofTaxus brevifolia from immature zygotic embryos via somatic embryogenesis is described. Embryogenic callus tissues were obtained by culturing immature zygotic embryos on Lloyd and McCown medium (MCM) supplemented with 160 μM 2,4-dichlorophenoxyacetic ...
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Somatic embryos from callus of Sequoia sempervirens
Plant Cell Reports, 1988Compact calli with a potential for somatic embryogenesis were obtained from complete or split mature zygotic embryos or from cotyledons and hypocotyls of in vitro grown seedlings of Sequoia sempervirens. Somatic embryos which showed a typical bipolar structure, were formed together with adventitious buds.
F, Bourgkard, J M, Favre
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Towards normalization of soybean somatic embryo maturation
Plant Cell Reports, 2005Soybean (Glycine max L. Merrill) somatic embryos have been useful for assaying seed-specific traits prior to plant recovery. Such traits could be assessed more accurately if somatic embryos more closely mimicked seed development. Amino acid supplements, carbon source, and abscisic acid and basal salt formulations were tested in an effort to modify ...
M A, Schmidt +3 more
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Journal of Plant Physiology, 2002
Summary Converted somatic embryos of Corydalis yanhusuo showed direct development of somatic embryos in the region at the junction of cotyledonary leaf and root when cultured on Murashige and Skoog (MS) basal medium supplemented with 0-16 mg/L abscisic acid (ABA) and with 2 mg/L being optimum for further development.
Chao-Lin Kuo +5 more
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Summary Converted somatic embryos of Corydalis yanhusuo showed direct development of somatic embryos in the region at the junction of cotyledonary leaf and root when cultured on Murashige and Skoog (MS) basal medium supplemented with 0-16 mg/L abscisic acid (ABA) and with 2 mg/L being optimum for further development.
Chao-Lin Kuo +5 more
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Cryopreservation of Somatic Embryos
1995Since somatic embryos (SEs) can be produced in large numbers in bioreactors (Denchev et al. 1992), and are being employed for mass micropropagation (Merkle et al. 1991; Bajaj 1991a), genetic transformation (Ellis 1993), and for the production of synthetic seed (Redenbaugh et al.
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