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Viability and Germination of the Pollen of Sorghum [Sorghum bicolor (L.) Moench]
Annals of Botany, 1994Previous studies have demonstrated that pollen of sorghum [Sorghum bicolor (L.) Moench] loses capacity to both germinate in vitro and to set seed in vivo soon after being shed. The current study evaluates the capacity for dehydrated pollen to effect in vitro germination, reduce tetrazolium chloride, and set seed on cytoplasmic male sterile plants ...
A R, Lansac +3 more
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DNA polymorphisms in grain sorghum (Sorghum bicolor (L.) Moench)
Theoretical and Applied Genetics, 1993Molecular markers [random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP)] were used to determine the frequency of DNA polymorphism in grain sorghum (Sorghum bicolor (L.) Moench). Twenty-nine oligonucleotide primers were employed for RAPDs, generating a total of 262 DNA fragments, of which 145 were polymorphic in at
Y, Tao +3 more
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Methods in molecular biology (Clifton, N.J.), 2006
This chapter describes a stepwise protocol for Agrobacterium-mediated sorghum genetic transformation. Immature embryos from sorghum plants were used as the target explants. The Agrobacterium strain LBA4404, carrying a "super-binary" vector, was used in this protocol.
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This chapter describes a stepwise protocol for Agrobacterium-mediated sorghum genetic transformation. Immature embryos from sorghum plants were used as the target explants. The Agrobacterium strain LBA4404, carrying a "super-binary" vector, was used in this protocol.
openaire +1 more source