Results 101 to 110 of about 2,690,144 (332)

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

open access: yesFEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield   +3 more
wiley   +1 more source

Application of a very detailed soil survey method in viticultural zoning in Catalonia, Spain

open access: yesOENO One, 2009
Aims: The aim of this study was to implement a very detailed soil survey methodology in 1,243 ha of vineyards in Catalonia (Spain) and analyse its suitability for viticultural zoning. Methods and results: The Soil Taxonomy at series level was used as the
Josep Miquel Ubalde   +2 more
doaj   +1 more source

Redox‐dependent binding and conformational equilibria govern the fluorescence decay of NAD(P)H in living cells

open access: yesFEBS Letters, EarlyView.
In this work, we reveal how different enzyme binding configurations influence the fluorescence decay of NAD(P)H in live cells using time‐resolved anisotropy imaging and fluorescence lifetime imaging microscopy (FLIM). Mathematical modelling shows that the redox states of the NAD and NADP pools govern these configurations, shaping their fluorescence ...
Thomas S. Blacker   +8 more
wiley   +1 more source

El proteccionismo de fin de siglo

open access: yesStudia Historica: Historia Contemporánea, 2010
RESUMEN: Dentro del análisis de la política comercial de la Europa continental, este trabajo profundiza en la reacción proteccionista que, contrastando con la apertura anterior, caracteriza el final del siglo XIX y los primeros años del XX, justo hasta ...
Marcela SABATÉ SORT
doaj  

Implementasi Topological Sort dalam Pengalihan Arus Kendaraan untuk Mengatasi Problem Kemacetan Lalu Lintas

open access: yesJournal Focus Action of Research Mathematic, 2018
Kemacetan lalu lintas merupakan hal yang sering terjadi di Kota Malang khususnya di pusat-pusat keramaian dan kawasan edukasi seperti di Jalan Bandung dan Jalan Veteran.
Nur Fadilatul Ilmiyah   +2 more
doaj   +1 more source

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

open access: yesFEBS Letters, EarlyView.
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan   +2 more
wiley   +1 more source

C‐mannosylation promotes ADAMTS1 activation and secretion in human testicular germ cell tumor NEC8 cells

open access: yesFEBS Letters, EarlyView.
C‐mannosylation is a unique form of protein glycosylation. In this study, we demonstrated that ADAMTS1 is C‐mannosylated at Trp562 and Trp565 in human testicular germ cell tumor NEC8 cells. We found that C‐mannosylation of ADAMTS1 is essential for its secretion, processing, enzymatic activity, and ability to promote vasculogenic mimicry. These findings
Takato Kobayashi   +5 more
wiley   +1 more source

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