Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Study of Human Papillomavirus Vaccine-Related Social Media Content Across Eight Social Media Platforms: Scoping Review of Communication Content, Information Sources, and Analytical Approaches. [PDF]
Xu Y +8 more
europepmc +1 more source
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta +7 more
wiley +1 more source
HPV Vaccine Uptake, Hesitancy and Information Sources Among Young Adults: A Cross-Sectional Study of Korea and the US. [PDF]
Jo S, Xu D, Song H.
europepmc +1 more source
Association between the food environment and the risk of ischemic stroke. [PDF]
Becker CJ +9 more
europepmc +1 more source
Soil polycyclic aromatic hydrocarbons in energy chemical industrial plants of Northwest China: Sources and health risk implications from a field-based study. [PDF]
Wu M +9 more
europepmc +1 more source
Preferred Sources of Health-Risk Information About Cigarettes, e-Cigarettes, and Waterpipes Among Adolescents in Germany: A Cross-Sectional Analysis of a Large School-Based Survey. [PDF]
Klosterhalfen S +4 more
europepmc +1 more source

