Rethinking Symptom Fluctuation in Multimorbidity: A Dynamic Systems Framework From Primary Care. [PDF]
J Eval Clin PractMatsuoka F.
europepmc +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Glial syncytial control fields and the low-frequency resonome of the human brain. [PDF]
Front Hum NeurosciBallús Santacana A.
europepmc +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Teachers and Students Investigating Plants in Space
In this activity students will grow "AstroPlants," a special fast-growing plant used in Space Shuttle microgravity experiments. They will become familiar with the growth process and life cycle of the plants and investigate the effects of gravity and ...
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Retrieval-Based Evaluation of Cell Painting Feature Spaces Reveals Differences in the Preservation of Biologically Meaningful Phenotypic Similarity. [PDF]
Int J Mol SciKuznetsova X +4 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Nature relatedness, nature-based physical activity, and positive mental health among Chinese university students: a three-wave longitudinal study. [PDF]
Front PsycholLiu S, Tan Y, Zang L.
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
An Educational Introduction to ITAR and EAR Regarding Pressure Suits and Space Suits
Immediate accessThis item is made available by the University of Arizona Center for Human Space Exploration (CHaSE) with support from the University of Arizona Libraries.
Tresch, Trent
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