Results 191 to 200 of about 1,706,547 (267)

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Cause and consequences of Common Snook (Centropomus undecimalis) space use specialization in a subtropical riverscape. [PDF]

Sci Rep
Santos RO, White M, James WR, Viadero NM, Massie JA, Boucek RE, Rehage JS.   +6 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Hidden space use behaviors of a nonbreeding migratory bird: the role of environment and social context. [PDF]

Mov Ecol
Dossman BC, Rodewald AD, Marra PP.
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

A hypergraph model shows the carbon reduction potential of effective space use in housing. [PDF]

Nat Commun
Weber RE, Mueller C, Reinhart C.
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Human Footprint and Forest Disturbance Reduce Space Use of Brown Bears (Ursus arctos) Across Europe. [PDF]

Glob Chang Biol
Hertel AG, Parres A, Frank SC, Renaud J, Selva N, Zedrosser A, Balkenhol N, Maiorano L, Fedorca A, Dutta T, Bogdanović N, Bragalanti N, Chiriac S, Ćirović D, Ciucci P, Domokos C, Fedorca M, Filacorda S, Finďo S, Groff C, de Gabriel Hernando M, Huber D, Ionescu G, Jerina K, Karamanlidis AA, Kindberg J, Kojola I, Mertzanis Y, Palazon S, Pop MI, Psaralexi M, Quenette PY, Sergiel A, Skuban M, Zlatanova D, Zwijacz-Kozica T, De Barba M.   +36 more
europepmc   +1 more source

Risk Prediction Models for Recurrence After Curative Treatment of Early‐Stage or Locally Advanced Lung Cancer: A Systematic Review

Aging and Cancer, EarlyView.
This systematic review synthesizes prognostic models for survival and recurrence in resected non‐small cell lung cancer. While many models demonstrate moderate to good discrimination, few are externally validated and reporting quality is variable, limiting clinical applicability and highlighting the need for robust, transparent model development ...
Evangeline Samuel, S. Tissera, E. Paul, J. Zalcberg, R. G. Stirling   +4 more
wiley   +1 more source

Integrating human mobility and animal movement data reveals complex space-use between humans and white-tailed deer in urban environments. [PDF]

Sci Rep
Péter SA, Gallo T, Mullinax J, Roess A, Palomo-Munoz G, Anderson T.   +5 more
europepmc   +1 more source