Results 241 to 250 of about 1,688,232 (308)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Early childhood caries in Romania: non-linear and spatial analysis of geographic sugar intake using LOESS, GAMs, and choropleth mapping. [PDF]

Front Oral Health
Sava-Rosianu R, Campus GG, Alexa VT, Ilin A, Postolache M, Jumanca D, Oancea R, Sfeatcu R, Caramida M, Muntean A, Esian D, Murariu A, Daguci C, Balean O, Bolchis V, Buzatu BLR, Luca D, Toderas N, Galuscan A.   +18 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Regional disparities in breast cancer mortality in Brazil: a spatial analysis using uncorrected and adjusted data, 2000-2023. [PDF]

Sci Rep
de Araújo Santos Camargo JD, Camargo SF, Camargo SF, de Souza ATB, de Oliveira Freitas AKMS, Ferreira CA, Sarmento ACA, de Oliveira Crispim JC, Gonzaga MR, Meira KC, Piuvezam G, Gonçalves AK.   +11 more
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Diffusion and division: A spatial analysis of surrogacy policy determinants in the United States. [PDF]

SSM Popul Health
Gao J, Idris MA, Gallegos GA, Griffin BC, Munroe SV, Windett JH.   +5 more
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Household Water and Sanitation Practices and Their Association With Diarrheal and Acute Respiratory Infections Among Children Under Five in Ghana: A Cross-Sectional Predictive Model and Spatial Analysis. [PDF]

Health Sci Rep
Salifu Y, Gyan P, Lasong J, Walana W.
europepmc   +1 more source

Disability-adjusted life years of clonorchiasis in China: a high-resolution spatial analysis. [PDF]

Infect Dis Poverty
Qian MB, Wang L, Huang JL, Zhou CH, Zhu TJ, Zhou XN, Lai YS, Li SZ.   +7 more
europepmc   +1 more source