Results 191 to 200 of about 26,522,155 (308)

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

From pledges to places: action agendas need spatial data to integrate climate and biodiversity action. [PDF]

NPJ Clim Action
Hagenström P, Pettorelli N, Boran I, Bridgewater P, Pugley DD, Folkard-Tapp H, Hsu A, Imbach P, Kok MTJ, VanDeveer SD, Widerberg O, Chan S.   +11 more
europepmc   +1 more source

Ocean Sciences with the Spilhaus Projection: A Seamless Ocean Map for Spatial Data Recognition. [PDF]

Sci Data, 2023
Chen J, Zhang T, Tominaga M, Escartin J, Kang R.   +4 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Equity in health resource allocation in Guangdong Province, China, from 2013-2021: research based on the Dagum Gini coefficient and exploratory spatial data analysis. [PDF]

Cost Eff Resour Alloc
Su W, Guan Z, Li X, Chen F, Guo P, Yuan C, Wang P.   +6 more
europepmc   +1 more source

The potential of small-scale spatial data in regional science. [PDF]

Jahrb Reg Wiss, 2022
Bergs R, Budde R.
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Mapping Community Vulnerability to reduced Vaccine Impact in Uganda and Kenya: A spatial Data-driven Approach. [PDF]

NIHR Open Res
Nalwanga R, Natukunda A, Zirimenya L, Chi P, Luzze H, Elliott AM, Kaleebu P, Trotter CL, Webb EL.   +8 more
europepmc   +1 more source

sptotal: an R package for predicting totals and weighted sums from spatial data. [PDF]

J Open Source Softw, 2023
Higham M, Hoef JV, Frank B, Dumelle M.
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source