Results 51 to 60 of about 13,138,760 (291)
Reconstructing enzyme evolution by protein engineering
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Silver birch (Betula pendula Roth) is a widely distributed, early-successional broadleaved species with ecological and partly also economic significance in Europe.
Konôpka Bohdan, Pajtík Jozef
doaj +1 more source
Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research
What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
wiley +1 more source
Leaf nutrient and specific leaf area data
Mass- and area-based nitrogen and phosphorus content, and specific leaf area of the dominant tree species at Daxiagu and Tianlongshan.
Dayong Fan (398329) +4 more
core +1 more source
Taxanes are widely used chemotherapeutics whose effects on cellular mechanics remain poorly understood. We show that paclitaxel induces rapid cellular contraction by promoting GEF‐H1 dissociation from microtubules and non‐muscle myosin II activation through RhoA/ROCK.
Gloria Asensio‐Juárez +5 more
wiley +1 more source
Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley +1 more source
It is important to understand how eco-physiological characteristics shift in forests when elucidating the mechanisms underlying species replacement and the process of succession and stabilization.
Yihua Xiao +5 more
doaj +1 more source
Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow +12 more
wiley +1 more source
Measurement of specific leaf area
Leaf specific surface area (SLA) plays a key role in many studies on plants, providing valuable information on their growth and adaptation strategies. Its measurement follows a well-defined process, which includes leaf sampling, acquisition and analysis of digital images, and subsequent data processing.
Latilla, Leonardo +2 more
openaire +2 more sources
Specific leaf area (SLA) of plants (cm2 g−1 DW) exposed to different soils collected in Ervedosa mine area and to REF and OECD artificial soil. The error bars represent the standard deviation and * correspond to significant differences towards the REF ...
Sara C. Antunes (397106) +6 more
core +1 more source

