Results 181 to 190 of about 5,643,837 (365)

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

NMR screening of low molecular weight inhibitors targeting the papain‐like protease (PLPro) of SARS‐CoV‐2

FEBS Open Bio, EarlyView.
Papain‐like protease from SARS‐CoV‐2 plays an important role in the cleavage of the viral polyproteins and in the suppression of the host's immune response. Here, we present the results of an NMR screening study. We identified 86 binding compounds, of which five candidates were chosen for in‐depth analysis.
Dennis J. Pyper, Sridhar Sreeramulu, Benjamin T. Lanham, Elizabeth M. Engle, David Fushman, Harald Schwalbe   +5 more
wiley   +1 more source

ALMA High-resolution Spectral Survey of Thioformaldehyde (H2CS) toward Massive Protoclusters

The Astrophysical Journal
Investigating the temperature and density structures of gas in massive protoclusters is crucial for understanding the chemical properties therein. In this study, we present observations of the continuum and thioformaldehyde (H _2 CS) lines at 345 GHz of ...
Li Chen, Sheng-Li Qin, Tie Liu, Hong-Li Liu, Sheng-Yuan Liu, Meizhu Liu, Hongqiong Shi, Chuanshou Li, Mengyao Tang, Tianwei Zhang, Ken’ichi Tatematsu, Xiaohu Li, Fengwei Xu, Yuefang Wu, Dongting Yang   +14 more
doaj   +1 more source

On the Theory of Spectral Line Widths of Coupled Many Wave Systems in Non-Thermal Equilibrium [PDF]

, 1974
W. Wonneberger
openalex   +1 more source

CD9‐association with PIP2 areas is regulated by a CD9 salt bridge

FEBS Open Bio, EarlyView.
The tetraspanin CD9 has an intracellular salt bridge. If CD9 opens, open‐CD9 moves from PIP2‐rich areas to regions populated by its interaction partner EWI‐2. Hence, the state of the salt bridge regulates the distribution of CD9 and by this CD9‐EWI‐2 complex formation.
Yahya Homsi, Sara C. Konopka, Thorsten Lang   +2 more
wiley   +1 more source

On the variation of intensity of spectral line with the difference of crystal structure (Part I)

, 1960
Chujo Matsumoto
openalex   +2 more sources

Spectral Line Observations of the Galactic Centre Region [PDF]

, 1977
R. D. Davies, R. J. Cohen
openalex   +1 more source

Nitric oxide‐forming nitrite reductases in the anaerobic ammonium oxidizer Kuenenia stuttgartiensis

FEBS Open Bio, EarlyView.
Anammox bacteria remove fixed nitrogen from their environment via anaerobic ammonium oxidation (anammox) with nitrite as electron acceptor and dinitrogen gas as product. The first anammox step is the conversion of nitrite to nitric oxide by nitrite reductase.
Femke J. Vermeir, Lotte W. Nijman, Robert S. Jansen, Laura van Niftrik, Wouter Versantvoort   +4 more
wiley   +1 more source

Spectral Line Formation in YY Ori and T Tau Envelopes [PDF]

, 1977
C. Bertout
openalex   +1 more source

Most autophagic cell death studies lack evidence of causality

FEBS Open Bio, EarlyView.
Of 104 studies claiming autophagic cell death (ACD), only 13 demonstrated both causality and exclusion of apoptosis to confirm true ACD. Most studies relied on correlation‐level data or measured autophagy in isolation, revealing pervasive methodological shortcomings.
Ali Burak Özkaya, Yasmin Ghaseminejad
wiley   +1 more source