Results 51 to 60 of about 6,533 (191)

Real‐Time Multicolor Fluorescence Microscopy via Cross‐Channel Acquisition and Deep‐Learning‐Based Inference

open access: yesAdvanced Intelligent Discovery, EarlyView.
Sequential multicolor fluorescence imaging in dynamic microsystems is constrained by acquisition speed and excitation dose. This study introduces a real‐time framework to reconstruct spectrally separated channels from reduced cross‐channel acquisitions (frames containing mixed spectral contributions).
Juan J. Huaroto   +3 more
wiley   +1 more source

ASSESSING AND COMPARING THE PERFORMANCE OF ENDMEMBER EXTRACTION METHODS IN MULTIPLE CHANGE DETECTION USING HYPERSPECTRAL DATA [PDF]

open access: yesThe International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences, 2019
Endmember extraction is a process to identify the hidden pure source signals from the mixture. Endmember finding has become increasingly important in hyperspectral data exploitation because endmembers can be used to specify unknown particular spectral ...
H. Jafarzadeh, M. Hasanlou
doaj   +1 more source

UNMIX-ME: spectral and lifetime fluorescence unmixing via deep learning

open access: yesBiomedical Optics Express, 2020
Hyperspectral fluorescence lifetime imaging allows for the simultaneous acquisition of spectrally resolved temporal fluorescence emission decays. In turn, the acquired rich multidimensional data set enables simultaneous imaging of multiple fluorescent species for a comprehensive molecular assessment of biotissues.
Jason T. Smith   +2 more
openaire   +2 more sources

Overcoming the Nyquist Limit in Molecular Hyperspectral Imaging by Reinforcement Learning

open access: yesAdvanced Intelligent Discovery, EarlyView.
Explorative spectral acquisition guide automatically selects informative spectral bands to optimize downstream tasks, outperforming full‐spectrum acquisition. The selected hyperspectral data are used for tasks such as unmixing and segmentation. BandOptiNet encodes selection states and outputs optimal bands to guide spectral acquisition. Recent advances
Xiaobin Tang   +4 more
wiley   +1 more source

Fluorescent Diarylethenes With Polar Groups: Synthesis, Spectra, and Optical Microscopy Applications

open access: yesChemistry – A European Journal, EarlyView.
Blinking green and flashing red: photo‐switchable and photo‐activatable probes emitting green and red light were prepared and applied as bioconjugates in super‐resolution optical microscopy. ABSTRACT The use of photoactivatable fluorescent diarylethenes (fDAEs) in biology‐related light microscopy has been restricted by the lack of probes having freely ...
Kakishi Uno   +6 more
wiley   +1 more source

Measurement and Prediction of Unmixing‐Dependent Spreading due to Collinearity in Spectral Flow Cytometry

open access: yesCytometry Part A, EarlyView.
ABSTRACT Advances in spectral cytometry instrumentation and fluorescent reagents have led to the possibility of ultra‐high‐parameter panels exceeding 50 colors. However, panel size is limited in practice by unmixing‐dependent spreading (UDS), a phenomenon which leads to a progressive deterioration of unmixed signal‐to‐noise ratios in panels that ...
Peter L. Mage   +2 more
wiley   +1 more source

Research on the Hyperspectral-based Unmixing Model of Composite Pigments on Mural Surfaces [PDF]

open access: yesThe International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences
Murals constitute an invaluable component of cultural heritage, encapsulating profound artistic and historical significance. Hyperspectral remote sensing, as a non-destructive testing technique, offers an effective means for analysing and identifying ...
Y. Zhang   +8 more
doaj   +1 more source

A New Deep Convolutional Network for Effective Hyperspectral Unmixing

open access: yesIEEE Journal of Selected Topics in Applied Earth Observations and Remote Sensing, 2022
Hyperspectral unmixing extracts pure spectral constituents (endmembers) and their corresponding abundance fractions from remotely sensed scenes. Most traditional hyperspectral unmixing methods require the results of other endmember extraction algorithms ...
Xuanwen Tao   +7 more
doaj   +1 more source

Nanocubes and Cuboctahedra: Entropic Stabilization Versus Thermal Segregation in Rock Salt‐ and Spinel‐Type Multinary Oxides from Spray‐Flame Synthesis

open access: yesENERGY &ENVIRONMENTAL MATERIALS, EarlyView.
Spray‐flame synthesis enables highly flexible and scalable production of multinary and high‐entropy oxide nanoparticles due to high synthesis temperatures with subsequent quenching. Temperature‐dependent in situ diffraction of as‐synthesized materials clearly reveals entropy stabilization in rock salt lattices, while spinels—featuring tetrahedral and ...
Mohammed‐Ali Sheikh   +7 more
wiley   +1 more source

Graph-Based Adaptive Network With Spatial-Spectral Features for Hyperspectral Unmixing

open access: yesIEEE Journal of Selected Topics in Applied Earth Observations and Remote Sensing
Hyperspectral unmixing aims to extract basic material (endmember) spectra and estimate their corresponding fractions (abundances) from observed pixels in hyperspectral images (HSIs).
Hua Dong   +4 more
doaj   +1 more source

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