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Taxonomy and Identification of Spiroplasmas
Clinical Infectious Diseases, 1982Within the past decade a new genus and family of helical, wall-less prokaryotes have been recognized. To date, eight groups have been described, four of which represent species or putative species. Methods for rapid identification or confirmation of these serovars have been devised.
Joseph G. Tully, Robert F. Whitcomb
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Molecular detection of Spiroplasma apis and Spiroplasma melliferum in bees
Journal of Invertebrate Pathology, 2012Spiroplasma apis and Spiroplasma melliferum are known as honey bee pathogens and are detected by unspecific methodologies like culturing or dark field microscopy. We developed a multiplex PCR being able to differentiate between both species and detect the genus Spiroplasma. This PCR can directly be used on culture samples or on DNA extracted bees.
Ivan Meeus +2 more
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Annual Review of Entomology, 1981
Since their discovery as members of a new microbial taxon in 1972 (40), spiroplasmas (5, 7, 33, 36, 39, 145, 149) have been investigated intensively as microbes. They are, after all, unique among wall-less microorganisms in their capacity to maintain helical shape and to exhibit several types of motility (including translational movement) in viscous ...
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Since their discovery as members of a new microbial taxon in 1972 (40), spiroplasmas (5, 7, 33, 36, 39, 145, 149) have been investigated intensively as microbes. They are, after all, unique among wall-less microorganisms in their capacity to maintain helical shape and to exhibit several types of motility (including translational movement) in viscous ...
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Viruses of Mycoplasmas and Spiroplasmas
1977Publisher Summary The term mycoplasma refers to a group of microorganisms (Class Mollicutes, Order Mycoplasmatales), previously known as pleuropneumonia-like organisms or PPLO. The many different isolates have been cataloged into the genera mycoplasma, acholeplasma, ureaplasma or T-strains, spiroplasma, thermoplasmn, and anaeroplasma. Mycoplasmas are
Jack Maniloff +2 more
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Characterization of Spiroplasmas by Serology
Clinical Infectious Diseases, 1982Five major serologic groups of spiroplasmas have been distinguished on the basis of serology (as determined in a test of deformation and metabolism inhibition), guanine-plus-cytosine content of DNA, and DNA-DNA hybridization. group I includes strains of Spiroplasma citri that form a classical subgroup (serovar) complex of four serologically homogeneous
David L. Williamson, Joseph G. Tully
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Pathogenicity of Spiroplasma apis and other spiroplasmas for honey-bees in Southwestern France
Annales de l'Institut Pasteur / Microbiologie, 1984The haemolymph of honey-bees affected by a May disease-like disorder in southwestern France contained numerous spiroplasmas. Further characterization of the organisms and pathogenicity assays showed that the causal agent of the disease was a spiroplasma belonging to group IV. The name Spiroplasma apis was given to the reference strain B31 (ATCC 33834),
Joseph M. Bové +5 more
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1987
Spleen cells from mice immunized with a mixture of S. citri (M200H) and three strains of corn stunt spiroplasma (F32, PU8–17, I747) were fused with NSI myelomas for the production of monoclonal antibody-secreting hybridomas. Hybridoma supernatants were initially screened against the mixture of spiroplasmas in heterologous double-antibody sandwich ...
M. Konai +4 more
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Spleen cells from mice immunized with a mixture of S. citri (M200H) and three strains of corn stunt spiroplasma (F32, PU8–17, I747) were fused with NSI myelomas for the production of monoclonal antibody-secreting hybridomas. Hybridoma supernatants were initially screened against the mixture of spiroplasmas in heterologous double-antibody sandwich ...
M. Konai +4 more
openaire +2 more sources