Spoligotyping of the Mycobacterium tuberculosis complex using on-Chip PCR
Abstract Aims The aim of this study was to develop a rapid PCR-based method for spoligotyping of Mycobacteria in the microarray format and to compare it to conventional spoligotyping by hybridization.
Sergey, Lapa+3 more
openaire +3 more sources
SpoTyping: fast and accurate in silico Mycobacterium spoligotyping from sequence reads [PDF]
SpoTyping is a fast and accurate program for in silico spoligotyping of Mycobacterium tuberculosis isolates from next-generation sequencing reads. This novel method achieves high accuracy for reads of both uniform and varying lengths, and is about 20 to 40 times faster than SpolPred.
Xia E., Teo Y.-Y., Ong R.T.-H.
openaire +3 more sources
Following infection, bacterial killing by polymorphonuclear leukocytes (neutrophils) is the main host defense against bacteria. Our hypothesis is that particles of ultra-high molecular weight polyethylene (UHMWP) may impair local neutrophil function and ...
Bernard, Louis+6 more
core +2 more sources
Genetic characterization of Mycobacterium tuberculosis in the West Bank, Palestinian Territories [PDF]
BACKGROUND: The World Health Organization (WHO) declared human tuberculosis (TB) a global health emergency and launched the “Global Plan to Stop Tuberculosis” which aims to save a million lives by 2015.
Abedelmajeed Nasereddin+7 more
core +1 more source
Spoligotyping of Mycobacterium tuberculosis isolates using Luminex®-based method in Lebanon
Introduction: Data about the genotypes of circulating Mycobacterium tuberculosis isolates (MTB) in Lebanon are scarce. This study was undertaken to reveal the spoligotypes of MTB isolates recovered from patients in Lebanon. Methodology: MTB isolates from 49 patients living in Lebanon were recovered and identified. The samples were heat killed and
Masoud, Khaldoun+11 more
openaire +6 more sources
During a population-based study to genotype isolates of Mycobacterium tuberculosis from Buenos Aires Northern suburbs, we found isolates with molecular patterns related to those of the Beijing genotype.
N. Morcillo+9 more
doaj
Tuberculosis in Swiss captive Asian elephants: microevolution of Mycobacterium tuberculosis characterized by multilocus variable-number tandem-repeat analysis and whole-genome sequencingle-number tandem-repeat analysis and whole-genome sequencing [PDF]
Zoonotic tuberculosis is a risk for human health, especially when animals are in close contact with humans. Mycobacterium tuberculosis was cultured from several organs, including lung tissue and gastric mucosa, of three captive elephants euthanized in a ...
Coscolla, Mireia+8 more
core +3 more sources
The purpose of this study was to provide information about the genetic diversity and prevalent genotype of Mycobacterium tuberculosis in a low-endemic setting in northwestern state of Paraná in Southern Brazil. We employed spoligotyping and mycobacterial
Erika Noda Noguti+7 more
doaj +1 more source
A Multinational Analysis of Mutations and Heterogeneity in PZase, RpsA, and PanD Associated with Pyrazinamide Resistance in M/XDR Mycobacterium tuberculosis. [PDF]
Pyrazinamide (PZA) is an important first-line drug in all existing and new tuberculosis (TB) treatment regimens. PZA-resistance in M. tuberculosis is increasing, especially among M/XDR cases.
Catanzaro, A+7 more
core +1 more source
Objective Based on our recent studies the prevalence of polyclonal infection in tuberculosis clinical specimens is more than 50% in Tehran, Iran. With this background, Spoligotyping was performed on clinical specimens and their respective cultures, and ...
Mansour Kargarpour Kamakoli+7 more
doaj +1 more source