Spoligotyping andMycobacterium tuberculosis [PDF]
We evaluated the clinical usefulness of spoligotyping, a polymerase chain reaction-based method for simultaneous detection and typing of Mycobacterium tuberculosis strains, with acid-fast bacilli-positive slides from clinical specimens or mycobacterial cultures. Overall sensitivity and specificity were 97% and 95% for the detection of M.
A. Gori+11 more
doaj +7 more sources
Spoligotype Database of Mycobacterium tuberculosis: Biogeographic Distribution of Shared Types and Epidemiologic and Phylogenetic Perspectives [PDF]
We give an update on the worldwide spoligotype database, which now contains 3,319 spoligotype patterns of Mycobacterium tuberculosis in 47 countries, with 259 shared types, i.e., identical spoligotypes shared by two or more patient isolates.
Christophe Sola+5 more
doaj +3 more sources
Mycobacterium tuberculosis Spoligotypes in Monterrey, Mexico [PDF]
ABSTRACT Although tuberculosis is still a public health problem in Mexico, there is little information about the genetic characteristics of the isolates. In the present study, we analyzed by spoligotyping 180 Mycobacterium tuberculosis clinical isolates from the urban area of Monterrey, Mexico, including ...
Lucio Vera-Cabrera+8 more
openaire +4 more sources
Genetic Diversity of Mycobacterium tuberculosis in Sicily Based on Spoligotyping and Variable Number of Tandem DNA Repeats and Comparison with a Spoligotyping Database for Population-Based Analysis [PDF]
ABSTRACT In a previous study, we proposed to associate spoligotyping and typing with the variable number of tandem DNA repeats (VNTR) as an alternative strategy to IS 6110 -restriction fragment length polymorphism (RFLP) for molecular epidemiological studies on tuberculosis.
NASTASI, ANTONINO+4 more
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Molecular Characterisation of Mycobacterium bovis Isolates from Cattle Slaughtered in Adamawa and Gombe States, North-Eastern Nigeria [PDF]
Bovine tuberculosis is endemic in Nigeria with control measures as provided by the laws of the country being minimally enforced mostly at the abattoirs only. This study focused on bovine tuberculosis in Adamawa and Gombe States. Tuberculosis lesions were
Sadiq Mohammed Damina+9 more
doaj +2 more sources
A first insight into tuberculosis transmission at the border of Ecuador and Colombia: a retrospective study of the population structure of Mycobacterium tuberculosis in Esmeraldas province [PDF]
ObjectiveTuberculosis (TB) is a major public health concern in Ecuador and Colombia, considering that both countries are high-burden TB settings. Molecular epidemiology is crucial to understand the transmission dynamics of Mycobacterium tuberculosis ...
Bernardo Castro-Rodriguez+12 more
doaj +2 more sources
Models of deletion for visualizing bacterial variation: an application to tuberculosis spoligotypes [PDF]
Molecular typing methods are commonly used to study genetic relationships among bacterial isolates. Many of these methods have become standardized and produce portable data. A popular approach for analyzing such data is to construct graphs, including phylogenies.
Reyes, Josephine F+2 more
openaire +7 more sources
Comparative Mycobacterium tuberculosis Spoligotype Distribution in Mexico [PDF]
ABSTRACT In the present work, we studied the genetic diversity of Mycobacterium tuberculosis clinical isolates from patients according to their gender, age, and geographic location in Mexico. We did not observe any statistically significant differences in regard to age or gender.
Jessica Ramos-Alvarez+6 more
openaire +4 more sources
Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil [PDF]
We performed spoligotyping and 12-mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTRs) typing to characterise Mycobacterium bovis isolates collected from tissue samples of bovines with lesions suggestive for tuberculosis
Patrícia Martins Parreiras+11 more
doaj +2 more sources
Spoligotyping ofMycobacterium africanum, Burkina Faso
Using Ziehl-Neelsen-positive slides collected from tuberculosis diagnostic centers in Burkina Faso, we showed that 20% of 80 spoligotyping-positive DNA samples had a characteristic Mycobacterium africanum-specific genomic signature. This result suggests that M. africanum is still present in Burkina Faso at almost the same prevalence as 15-20 years ago.
Gomgnimbou, Michel K+6 more
openaire +6 more sources