Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Will the Construction of Sports Facilities Nudge People to Participate in Physical Exercises in China? The Moderating Role of Mental Health. [PDF]
Healthcare (Basel), 2023 Xue X, Li Y.
europepmc +1 more source
FEBS Open Bio, EarlyView.The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
The analysis effectiveness of management in A.Ratkeviciaus sports school. [PDF]
, 2013 The object of research: Management of A.Ratkeviciaus sports school The aim of work: to determine how effectively A.Ratkeviciaus sports school is being managed. The tasks of work: 1. To reveal theoretical side of ruling the sport facilities; 2. To analyze
Narkun, Marius,
core
Development of Evaluation Indicators for Sports Facilities for People with Disabilities Considering the Universal Design: Focusing on the Republic of Korea. [PDF]
Healthcare (Basel), 2022 Yi E, Jeon SW, Oh A.
europepmc +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Contingent Valuation of Sports Stadiums and Arenas: Temporal Embedding and Order Effect [PDF]
Using the Contingent Valuation Method, this paper estimates the value of public goods the National Football League’s Jaguars produce for Jacksonville, Florida, including the value of elevating Jacksonville to “major league” status and the value of ...
John C. Whitehead +2 more
core
Financing Professional Sports Facilities [PDF]
, 2011 This paper examines public financing of professional sports facilities with a focus on both early and recent developments in taxpayer subsidization of spectator sports.
Matheson, Victor, Baade, Robert A.
core +1 more source
An unsupervised machine learning approach to evaluate sports facilities condition in primary school. [PDF]
PLoS One, 2022 Xia J +5 more
europepmc +1 more source
FEBS Open Bio, EarlyView.KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura +19 more
wiley +1 more source