Results 171 to 180 of about 93,765 (263)
Structural complexity and groundwater circulation in mountain karst systems: an integrated approach combining hydrodynamic data and environmental tracers. [PDF]
Raibi F +7 more
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Instability detection and overload controlling system: a case of public transportation. [PDF]
Kahsay BG, Nallamothu RB.
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Predicting Activation Barriers With Ring and Bond Critical Points in the Kemp Elimination Reaction. [PDF]
Redmon H +3 more
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Unveiling spring behaviour through copula-based inter-spring connectivity in the Central Himalaya. [PDF]
Mukherjee S +3 more
europepmc +1 more source
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
Environmental Investigation of a Campylobacteriosis Outbreak Among Wedding Attendees - York County, Pennsylvania, 2025. [PDF]
Kamara RZ +14 more
europepmc +1 more source

