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Differential Staining of Bacteria: Gram Stain

Current Protocols in Microbiology, 2009
AbstractIn 1884, Hans Christian Gram, a Danish doctor, developed a differential staining technique that is still the cornerstone of bacterial identification and taxonomic division. This multistep, sequential staining protocol separates bacteria into four groups based on cell morphology and cell wall structure: Gram‐positive cocci, Gram‐negative cocci ...
Rita B. Moyes   +2 more
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Gram Staining [PDF]

open access: possibleCurrent Protocols in Microbiology, 1997
AbstractNamed after Hans Christian Gram who developed the method in 1884, the Gram stain allows one to distinguish between Gram‐positive and Gram‐negative bacteria on the basis of differential staining with a crystal violet‐iodine complex and a safranin counterstain.
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Staining Proteins in Gels [PDF]

open access: possibleCurrent Protocols Essential Laboratory Techniques, 2003
AbstractThis unit describes protocols for detecting proteins in SDS‐polyacrylamide gels. It describes methods for Coomassie blue and silver staining, as well as the fluorescent stains SYPRO orange and red. Staining with Coomassie blue is easier and more rapid; however, silver staining methods are considerably more sensitive and can thus be used to ...
Joachim Sasse, Sean R. Gallagher
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Stain Technology: Progress in the Standardization of Stains: The History of Staining

Stain Technology, 1932
(1932). Stain Technology: Progress in the Standardization of Stains: The History of Staining. Stain Technology: Vol. 7, No. 4, pp. 113-114.
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The Orcein Stain—A Versatile Stain for Histopathology

Journal of Histotechnology, 2002
Combinations of the orcein stain with other special stains that allow the demonstration of elastic tissue, basement membranes, collagen, ground substance, iron, and mucins in the same section are p...
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The Staining of Radulae

Stain Technology, 1948
A review of the various methods of staining and mounting radulae is given. Normally the radula should be extracted with 0.5 to 1% sodium hydroxide solution, and the associated tissues removed before staining. Two staining methods are recommended for facilitating the interpretation of radulae.
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A stain with catechol

Acta Histochemica, 1977
Summary When catechol, the simplest o-dihydroxy aromatic compound, is oxidized in the presence of potassium alum, there results a solution which stains very similarly to an alum hematoxylin.
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Stains, Staining and the Ethics of Dirty Work

2012
This chapter focuses on the theoretical potential of stains and staining as a productive way of understanding dirty work, especially the physical,social and moral taint attributed to it (Ashforth and Kreiner, 1999; Drew et al., 2007). The concept of the stain can be understood through the ethics of dirty work, focusing on healthcare and the presence of
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Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry

Nature Photonics, 2022
Daniele Pirone   +2 more
exaly  

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