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Preliminary Staining of Bacteria: Negative Stain
Current Protocols in Microbiology, 2009AbstractNegative staining is one of the many staining techniques that can be employed for viewing of bacterial cell morphology and size. The advantages of the negative stain include the use of only one stain and the absence of heat fixation of the sample.
Rita B, Moyes +2 more
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Immunoperoxidase staining of previously stained tissue
Journal of Cutaneous Pathology, 1984Using an unlabelled antibody peroxidase‐antiperoxidase method, We have demonstrated than certain tissue antigens can be identified in tissue sections previously stained with hematoxylin and eosin. This technique is useful in identifying antigens in tissues where insufficient unstained material is available.
D, Walder, N S, Penneys, M, Nadji
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Stain–Decolorize–Stain (SDS): a new technique for multiple staining
Histochemistry and Cell Biology, 2014Multiple staining of more than one gene/antigen on a single tissue section is an indispensable tool in cell and tissue research. However, most of the available multiple staining techniques have limitations, and there has been no technique to simultaneously visualize and distinguish tissue antigens, nucleotide sequences and other chemical compounds on ...
Jing, Li, Yan, Zhou, Jiang, Gu
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Differential Staining of Bacteria: Capsule Stain
Current Protocols in Microbiology, 2009AbstractBacterial capsules are composed of high‐molecular‐weight polysaccharides and/or polypeptides, and are associated with virulence and biofilm formation. Unfortunately, capsules do not stain well with crystal violet, methylene blue, or other simple stains. This unit describes two methods of capsule staining.
Donald P, Breakwell +2 more
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Differential Staining of Bacteria: Gram Stain
Current Protocols in Microbiology, 2009AbstractIn 1884, Hans Christian Gram, a Danish doctor, developed a differential staining technique that is still the cornerstone of bacterial identification and taxonomic division. This multistep, sequential staining protocol separates bacteria into four groups based on cell morphology and cell wall structure: Gram‐positive cocci, Gram‐negative cocci ...
Rita B, Moyes +2 more
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Current Protocols in Microbiology, 1997
AbstractNamed after Hans Christian Gram who developed the method in 1884, the Gram stain allows one to distinguish between Gram‐positive and Gram‐negative bacteria on the basis of differential staining with a crystal violet‐iodine complex and a safranin counterstain.
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AbstractNamed after Hans Christian Gram who developed the method in 1884, the Gram stain allows one to distinguish between Gram‐positive and Gram‐negative bacteria on the basis of differential staining with a crystal violet‐iodine complex and a safranin counterstain.
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Gastrointestinal Endoscopy Clinics of North America, 1994
Tissue staining has broad clinical and research application in gastroenterology but remains underused. New application and the development of novel "stains" should result in improved detection of gastrointestinal disease. Expanded research in tissue staining is needed and data on outcome effectiveness awaited.
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Tissue staining has broad clinical and research application in gastroenterology but remains underused. New application and the development of novel "stains" should result in improved detection of gastrointestinal disease. Expanded research in tissue staining is needed and data on outcome effectiveness awaited.
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Differential Staining of Bacteria: Acid Fast Stain
Current Protocols in Microbiology, 2009AbstractAcid‐fastness is an uncommon characteristic shared by the genera Mycobacterium (Section 10A) and Nocardia. Because of this feature, this stain is extremely helpful in identification of these bacteria. Although Gram positive, acid‐fast bacteria do not take the crystal violet into the wall well, appearing very light purple rather than the deep ...
Jackie, Reynolds +2 more
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Staining Juxtaglomerular Granules with Basic Fluorescent Stains
Stain Technology, 1969Many basic fluorescent dyes stain juxtaglomerular granules to produce characteristic colors in ultraviolet light. The stain is applied to paraffin sections of tissues fixed in 2% calcium acetate-10% formalin or in phosphate-buffered 10% formalin. Procedure: Bring section to water, stain 0.5 min in Delafield hematoxylin, wash in tap water, stain 3 min ...
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