A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast
We have used a lipophilic styryl dye, N-(3-triethylammoniumpropyl)-4- (p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM 4-64), as a vital stain to follow bulk membrane-internalization and transport to the vacuole in yeast.
T. Vida, S. Emr
semanticscholar +1 more source
Organoids in pediatric cancer research
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source
PEREMPUAN DAN PRODUK HUKUM YANG MENJAMIN KEADIILAN DAN KESETARAAN GENDER
Justice and Gender Equality or referred to KKG, a key word in every movement made by women to fight for their rights in various lines. This happens because in reality, women experience discrimination, marginalization, sub-ordinated, double burden, and ...
Tim PSG STAIN Pekalongan
doaj +1 more source
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley +1 more source
An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino +7 more
wiley +1 more source
A comparison of methodologies for the staining and quantification of intracellular components of Arbuscular Mychorrizal (AM) fungi in the root cortex of two varieties of winter wheat [PDF]
© 2019 The Authors. The definitive peer reviewed, edited version of this article is published in Access Microbiology, https://doi.org/10.1099/acmi.0.000083.
Davies, Keith +4 more
core +2 more sources
Stain Deconvolution Using Statistical Analysis of Multi-Resolution Stain Colour Representation
Stain colour estimation is a prominent factor of the analysis pipeline in most of histology image processing algorithms. Providing a reliable and efficient stain colour deconvolution approach is fundamental for robust algorithm. In this paper, we propose
Najah Alsubaie +4 more
semanticscholar +1 more source
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang +8 more
wiley +1 more source
O objetivo deste trabalho foi estimar a viabilidade do pólen em acessos de capim-elefante, milheto e híbridos interespecíficos por meio de germinação in vitro e coloração com carmim propiônico, orceína acética e corante de Alexander.
Antônio Vander Pereira +3 more
doaj

