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Stain–Decolorize–Stain (SDS): a new technique for multiple staining
Histochemistry and Cell Biology, 2014Multiple staining of more than one gene/antigen on a single tissue section is an indispensable tool in cell and tissue research. However, most of the available multiple staining techniques have limitations, and there has been no technique to simultaneously visualize and distinguish tissue antigens, nucleotide sequences and other chemical compounds on ...
Jing Li, Yan Zhou, Jiang Gu, Jiang Gu
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Preliminary Staining of Bacteria: Negative Stain [PDF]
Current Protocols in Microbiology, 2009 AbstractNegative staining is one of the many staining techniques that can be employed for viewing of bacterial cell morphology and size. The advantages of the negative stain include the use of only one stain and the absence of heat fixation of the sample.
Rita B. Moyes +2 more
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Differential Staining of Bacteria: Flagella Stain
Current Protocols in Microbiology, 2009AbstractBacterial flagella are appendages used for motility. Their presence is a useful tool for identification and differentiation of prokaryotes. Since flagella are too thin to be seen by compound light microscopy, staining methods employ the use of a mordant (often tannic acid) to make them thick enough to see using an oil immersion objective.
Rita B. Moyes +2 more
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Preliminary Staining of Bacteria: Simple Stains
Current Protocols in Microbiology, 2009AbstractSimple staining involves directly staining the bacterial cell with a positively charged dye in order to see bacterial detail, in contrast to negative staining where the bacteria remain unstained against a dark background. Curr. Protoc. Microbiol. 15:A.3E.1‐A.3E.5. © 2009 by John Wiley & Sons, Inc.
Jackie Reynolds +2 more
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Hematoxylin and eosin staining of tissue and cell sections.
Cold Spring Harbor Protocols, 2008INTRODUCTIONHematoxylin and eosin (H&E) stains have been used for at least a century and are still essential for recognizing various tissue types and the morphologic changes that form the basis of contemporary cancer diagnosis.
A. Fischer +3 more
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Differential Staining of Bacteria: Capsule Stain
Current Protocols in Microbiology, 2009AbstractBacterial capsules are composed of high‐molecular‐weight polysaccharides and/or polypeptides, and are associated with virulence and biofilm formation. Unfortunately, capsules do not stain well with crystal violet, methylene blue, or other simple stains. This unit describes two methods of capsule staining.
Jackie Reynolds +2 more
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Coffee stain on textiles. Mechanisms of staining and stain removal
Journal of the American Oil Chemists' Society, 1995AbstractCoffee stains on textiles are mainly caused by the water‐soluble and acidic colored substances in coffee. The acidic nature of coffee stain has been shown by ultraviolet and visible spectroscopy of coffee as a function of pH; ion‐pair formation with a cationic surfactant and titration with Hyamine 1622 and a surfactant‐specific electrode; and ...
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Immunoperoxidase staining of previously stained tissue
Journal of Cutaneous Pathology, 1984Using an unlabelled antibody peroxidase‐antiperoxidase method, We have demonstrated than certain tissue antigens can be identified in tissue sections previously stained with hematoxylin and eosin. This technique is useful in identifying antigens in tissues where insufficient unstained material is available.
D. Walder +2 more
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Differential Staining of Bacteria: Gram Stain
Current Protocols in Microbiology, 2009AbstractIn 1884, Hans Christian Gram, a Danish doctor, developed a differential staining technique that is still the cornerstone of bacterial identification and taxonomic division. This multistep, sequential staining protocol separates bacteria into four groups based on cell morphology and cell wall structure: Gram‐positive cocci, Gram‐negative cocci ...
Rita B. Moyes +2 more
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Current Protocols in Microbiology, 1997 AbstractNamed after Hans Christian Gram who developed the method in 1884, the Gram stain allows one to distinguish between Gram‐positive and Gram‐negative bacteria on the basis of differential staining with a crystal violet‐iodine complex and a safranin counterstain.
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