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Analyzing Cell Death by Nuclear Staining with Hoechst 33342.
Cold Spring Harbor Protocols, 2016The nuclei of healthy cells are generally spherical, and the DNA is evenly distributed. During apoptosis the DNA becomes condensed, but this process does not occur during necrosis. Nuclear condensation can therefore be used to distinguish apoptotic cells
L. C. Crowley, B. Marfell, N. Waterhouse
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Manual hematoxylin and eosin staining of mouse tissue sections.
Cold Spring Harbor Protocols, 2014The hematoxylin and eosin (H&E) stain is the standard used for microscopic examination of tissues that have been fixed, processed, embedded, and sectioned. It can be performed manually or by automation.
R. Cardiff, Claramae H. Miller, R. Munn
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Staining Proteins in Gels [PDF]
AbstractThis unit describes protocols for detecting proteins in SDS‐polyacrylamide gels. It describes methods for Coomassie blue and silver staining, as well as the fluorescent stains SYPRO orange and red. Staining with Coomassie blue is easier and more rapid; however, silver staining methods are considerably more sensitive and can thus be used to ...
Joachim Sasse, Sean R. Gallagher
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Stain Technology: Progress in the Standardization of Stains: The History of Staining
Stain Technology, 1932(1932). Stain Technology: Progress in the Standardization of Stains: The History of Staining. Stain Technology: Vol. 7, No. 4, pp. 113-114.
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Differential staining of aborted and nonaborted pollen.
Stain Technology, 1969A single staining solution was made by compounding it in the following order (dyes were from British Drug Houses): ethanol, 10 ml; 1% malachite green in 95% ethanol, 1 ml; distilled water, 50 ml; glycerol 25 ml; phenol, 5 gm; chloral hydrate, 5 gm; acid ...
M. P. Alexander
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The Orcein Stain—A Versatile Stain for Histopathology
Journal of Histotechnology, 2002Combinations of the orcein stain with other special stains that allow the demonstration of elastic tissue, basement membranes, collagen, ground substance, iron, and mucins in the same section are p...
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Stain Technology, 1948
A review of the various methods of staining and mounting radulae is given. Normally the radula should be extracted with 0.5 to 1% sodium hydroxide solution, and the associated tissues removed before staining. Two staining methods are recommended for facilitating the interpretation of radulae.
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A review of the various methods of staining and mounting radulae is given. Normally the radula should be extracted with 0.5 to 1% sodium hydroxide solution, and the associated tissues removed before staining. Two staining methods are recommended for facilitating the interpretation of radulae.
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Stains, Staining and the Ethics of Dirty Work
2012This chapter focuses on the theoretical potential of stains and staining as a productive way of understanding dirty work, especially the physical,social and moral taint attributed to it (Ashforth and Kreiner, 1999; Drew et al., 2007). The concept of the stain can be understood through the ethics of dirty work, focusing on healthcare and the presence of
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