From fix to fit into the autoptic human brains. [PDF]
Formalin-fixed, paraffinembedded (FFPE) human brain tissues are very often stored in formalin for long time. Formalin fixation reduces immunostaining, and the DNA/RNA extraction from FFPE brain tissue becomes suboptimal.
Lavezzi, A., Paradiso, B, Simonato, M
core +1 more source
Aims: To evaluate the effect of ultrasound-targeted simvastatin-loaded microbubble destruction (UTMD SV ) for alleviation of the progression of osteoarthritis (OA) in rabbits through modulation of the peroxisome proliferator-activated receptor (PPARγ ...
Xinwei Wang +9 more
doaj +1 more source
Evaluation of a Novel Anti-Mucin 1 (MUC1) Antibody (PankoMab) as a Potential Diagnostic Tool in Human Ductal Breast Cancer; Comparison with Two Established Antibodies [PDF]
Aim: PankoMab is a novel antibody that recognizes a tumor-specific epitope of Mucin 1 (MUC1). The aim of this study was the evaluation of PankoMab as a potential diagnostic tool and its comparison with two established antibodies against MUC1 in human ...
Dian, Darius +7 more
core +1 more source
Holographic virtual staining of individual biological cells
Significance We present a method for virtual staining for morphological analysis of individual biological cells based on stain-free digital holography, allowing clinicians and biologists to visualize and analyze the cells as if they have been chemically ...
Y. Nygate +9 more
semanticscholar +1 more source
A modified method for clearing, staining and mounting plant-inhabiting mites
A modified method for clearing, staining and mounting plant-inhabiting mites is proposed. With this method, all mites can be cleared with a single agent and mounted in a single medium.
Farid FARAJI, Frank BAKKER
doaj +1 more source
Improved methods for detection of β-galactosidase (lacZ) activity in hard tissue [PDF]
The ß-galactosidase gene (lacZ) of Escherichia coli is widely used as a reporter gene. The expression of lacZ can be detected by enzyme-based histochemical staining using chromogenic substrates such as 5-bromo-4-chloro-3-indolyl-ß-D: -galactoside (X-gal).
A Aguzzi +27 more
core +2 more sources
Colour masks in digital image analysis: A technique for modal analyses of quartz saturated plutonic rocks [PDF]
Mineral modes can be calculated quickly and accurately by using colour masks in scanned digital images of stained, quartz saturated plutonic rocks. The image analysis method for determining modal compositions is as accurate as manual point counting, and ...
B.A. Elliott
doaj +1 more source
Immunofluorescence Staining [PDF]
AbstractThis unit provides a protocol for indirect immunofluorescence, which is a method that provides information about the locations of specific molecules and the structure of the cell. Antibody molecules for a specific target molecule are exposed to the cell or tissue being investigated.
openaire +6 more sources
Propidium iodide staining underestimates viability of adherent bacterial cells
Combining membrane impermeable DNA-binding stain propidium iodide (PI) with membrane-permeable DNA-binding counterstains is a widely used approach for bacterial viability staining.
Merilin Rosenberg +2 more
semanticscholar +1 more source
Mucosubstances in the porcine gastrointestinal tract: Fixation, staining and quantification [PDF]
Mucins are of great interest in intestinal research and histochemical methods are often employed to identify them. Since it is in the nature of mucins that they are “hard to hold onto” once they come into contact with water, a frequently used medium in ...
Drewes, Barbara +3 more
core +1 more source

