Results 141 to 150 of about 11,437,124 (248)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

Spatial clustering method for geographic data [PDF]

open access: yes, 2002
In the process of visualizing quantitative spatial data, it is necessary to classify attribute values into some class divisions. In a previous paper, the author proposed a classification method for minimizing the loss of information contained in ...
Osaragi, Toshihiro
core  

Short-term effects of management intensity on the spatial structure and biodiversity of Camptotheca acuminata-Liquidambar formosana mixed plantations

open access: yesForest Systems
Aim of study: We aimed to 1) analyze the initial responses of stand spatial structure and understory biodiversity in Camptotheca acuminata Decne and Liquidambar formosana Hance mixed plantations to crop tree release (CTR) under varying thinning ...
Meng Zhang, Jing-Yong Cai, Yang Wang
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

Tree Regeneration Spatial Patterns in Ponderosa Pine Forests Following Stand-Replacing Fire: Influence of Topography and Neighbors

open access: yes, 2017
Shifting fire regimes alter forest structure assembly in ponderosa pine forests and may produce structural heterogeneity following stand-replacing fire due, in part, to fine-scale variability in growing environments.
Carolyn Sieg   +6 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Spatial distribution of ulin (Eusideroxylon zwageri Teijsm. & Binnend.) based on slope position and its stand structure in the forest area of tabalong district

open access: yes, 2020
Forest resources in Tabalong District has been degraded, proved by the fact that Ulin trees (Eusideroxylon zwageri) are difficult to be found. Ulin is in the status of endangered species, thus it must be protected and under the development efforts ...
SUYANTO, SUYANTO   +3 more
core  

Controlling Spatial Forest Structure with Spatial Simulation in Forest Management Planning: A Case Study from Turkey

open access: yes, 2015
Decision Support Systems (DSS) is widely used to develop spatially explicit forest management plans through the integration of spatial parameters. As a part of this study, a simulation-based spatial DSS, the ETCAP Simlasyon program was developed and ...
Baskent, Emin Zeki   +3 more
core   +1 more source

Modelling height to crown base using non-parametric methods for mixed forests in China

open access: yesEcological Informatics
The height to crown base (HCB) of a tree is a vital characteristic that reflects the self-thinning ability of a tree, and it is used to determine the crown size. and predict the crown recession rate. This study simulated the HCB of Spruce fir broadleaved
Zeyu Zhou   +9 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

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