Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
Earth-mass planets with He atmospheres in the habitable zone of Sun-like stars. [PDF]
Lammer H+6 more
europepmc +1 more source
A MERLIN survey of 4.7-GHz excited OH masers in star-forming regions [PDF]
Lisa Harvey-Smith, R. J. Cohen
openalex +1 more source
Interaction of class III cellobiose dehydrogenase with lytic polysaccharide monooxygenase
The activity of lytic polysaccharide monooxygenase (LPMO) is supported by its auxiliary enzyme cellobiose dehydrogenase (CDH). The catalytic activity of both enzymes is coupled by electron transfer and a cyclic cascade generating substrates for both enzymes – hydrogen peroxide for LPMO and oxidized and non‐oxidized cellobiose and cello‐oligosaccharide ...
Angela Giorgianni+4 more
wiley +1 more source
One-third of Sun-like stars are born with misaligned planet-forming disks. [PDF]
Biddle LI+4 more
europepmc +1 more source
VLBI observations of 6.7 and 12.2 GHz methanol masers toward high mass star-forming regions
V. Minier, R. S. Booth, J. E. Conway
openalex +1 more source
Hubble Space TelescopeObservations of Star-forming Regions in NGC 3994/3995 [PDF]
D. Eggers+4 more
openalex +1 more source
Matrix: a complex amalgam of structures and functions in tumor microenvironment
The matrix is a dynamic, intricate three‐dimensional mesh of biomolecules with both structural and functional properties. This review deals with the complexity of this ‘molecular amalgam’ in the tumor microenvironment and highlights its importance in the maintenance and evolution of tumors by describing certain matrix biomolecules, such as ...
Spyros S. Skandalis+3 more
wiley +1 more source
Predicting organoid morphology through a phase field model: Insights into cell division and lumenal pressure. [PDF]
Tanida S+10 more
europepmc +1 more source
Spectrophotometry of star forming regions in HII galaxies [PDF]
C. Kehrig, Eduardo Telles, F. Cuisinier
openalex +1 more source