Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Integrated Dynamic Modeling and Ground Test Validation for Spacecraft Micro-Vibration Suppression Considering Disturbance, Isolation, and Pointing Control. [PDF]
Wang H, Yan H, Tian L, Zang X, Zu Y.
europepmc +1 more source
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Phase-Retrieval Algorithm for Hololens Resolution Analysis in a Sustainable Photopolymer. [PDF]
Lloret T +3 more
europepmc +1 more source
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Sustainable electrochemical detection of benoxinate using MnO₂/MWCNT carbon paste electrode. [PDF]
Hashem HM +3 more
europepmc +1 more source
Protocol for measuring mitochondrial respiration in mature human adipocytes using the Seahorse XF analyzer. [PDF]
Broghammer H, Gebhardt C, Heiker JT.
europepmc +1 more source
Ionic liquid-regulated interfacial charge transport and asymmetric device architecture for high-performance electrochemiluminescence. [PDF]
Liu M +12 more
europepmc +1 more source
Optimal Sensor Placement via a POD-QR Framework for High-Fidelity 3D Temperature Field Reconstruction in Large-Scale Ultra-Low Temperature Chest Freezers. [PDF]
Chen Y +5 more
europepmc +1 more source
Active host control of the internal O<sub>2</sub> microenvironment in reef-building corals. [PDF]
Zhang Q +6 more
europepmc +1 more source

