Results 101 to 110 of about 18,289 (169)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Spin effects in superfluidity, neutron matter, and neutron stars. [PDF]

Sci Adv
Sedrakian A, Rau PB.
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Biomimetic hybrid bioactive glasses gelatin composite rescues alveolar socket healing from diabetic impairment. [PDF]

BMC Oral Health
Salem EM, El-Aziz AMA, Alghonemy WY, Hanafy RA.   +3 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

The neutrophil-to-lymphocyte ratio in Parkinson's disease: a systematic review and meta-analysis of its association with differential diagnosis, severity, and prognosis. [PDF]

Clin Park Relat Disord
Cabanillas-Lazo M, Montes-Baldarrago A, Uriol-Alvino S, Baltazar-Ñahui H, Pereda-Leon NB, Jimenez-Alvarez H, Mayta-Tovalino F.   +6 more
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

The abundances of carbon and nitrogen in the atmospheres of classical Be stars. [PDF]

Astrophys Space Sci
Peters GJ, Gayley KG, Rast RG, Vink JS, Drake JJ.   +4 more
europepmc   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Quality of Life in Women with Gynecologic Cancer and Coexisting Chronic Conditions: The Role of Cancer-Related Fatigue and Perceived Caregiver Support. [PDF]

Healthcare (Basel)
Bai J, Gui G, Chen A, Chen T, Zhang X, Liu Y, Zhou H, Gao J, Xu F, Wang Q, Pan N, Jin W, Jiang H.   +12 more
europepmc   +1 more source