Results 161 to 170 of about 21,274,755 (304)

Environmental volatility is associated with adaptive caution in visual search. [PDF]

PLoS Comput Biol
Qiu N, Allenmark F, Chen S, Müller HJ, Shi Z.   +4 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Single-Cell Whole-Transcriptome Detection Expands the Observable RNA Space for AI Virtual-Cell Models. [PDF]

Biosensors (Basel)
Liu X, Zhang B, Zou K, Wan R, Zhu Q.
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

A Conceptual Framework for Drift-Aware IMU-Based Gait Analysis Using Motion Capture References. [PDF]

Sensors (Basel)
Rezk E.
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

ESTIMATING REVENUE-CAPTURE POTENTIAL ASSOCIATED WITH PUBLIC AREA RECREATION [PDF]

A traditional contingent valuation approach and the “"trip response method"” were examined as potential techniques for measuring public area recreation revenue-capture potential.
Bergstrom, John C., Teasley, R. Jeff, Cordell, H. Ken   +2 more
core  

The stoichiometry trap in cysteine redox biology. [PDF]

Free Radic Biol Med
Carroll KS.
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Modular Solvent-Free Carbon Removal: A Solid-Sorbent Reactor for Sequential Direct Air Capture and Flue Gas Decarbonization. [PDF]

ACS Omega
Hong JS, Yu H, Jang JH, Jo YI, Lee MY, Kim J, Park S, Nam KT.   +7 more
europepmc   +1 more source