Results 61 to 70 of about 1,145,175 (361)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Globalization and the Fiscal Autonomy of the State [PDF]

open access: yesSSRN Electronic Journal, 2002
Public finance in less developed countries is the focal point - both as source and destination - of many of the dilemmas and conflicts posed by development. Revenue mobilization for the allocative, distributive and stabilization functions of the state is severely constrained by the narrowness and instability of the tax base.
openaire   +3 more sources

Autonomy and capacity: a state-centred approach to post-communist transition in Central Europe [PDF]

open access: yes, 2004
The paper examines state characteristics and their policy implications in five Central European post-communist countries. It argues that policies on macroeconomic stabilisation, privatisation and FDI had been shaped by state-society relations, which, in ...
Adam, Z.
core  

The skills required for transition to university and study in biological sciences: A student perspective

open access: yesFEBS Open Bio, EarlyView.
Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley   +1 more source

Liberal-Democratic States Should Privilege Parental Efforts to Instill Identities And Values [PDF]

open access: yes, 2017
Liberal-democratic states’ commitments to equality and personal autonomy have always proven problematic with respect to state regulation of relations between parents and children.
Robinson, Andrew M
core   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

open access: yesFEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee   +6 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Psychological Need Thwarting in the Sport Context: Assessing the Darker Side of Athletic Experience [PDF]

open access: yes, 2011
Research in self-determination theory (Ryan & Deci, 2002) has shown that satisfac­tion of autonomy, competence, and relatedness needs in sport contexts is associated with enhanced engagement, performance, and well-being. This article outlines the initial
Bartholomew, Kimberley   +3 more
core   +1 more source

Small RNA pathways in mammalian oocytes

open access: yesFEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

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