Results 61 to 70 of about 43,887 (283)

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Randomization-Based Confidence Intervals for Cluster Randomized Trials [PDF]

, 2020
In a cluster randomized trial (CRT), groups of people are randomly assigned to different interventions. Existing parametric and semiparametric methods for CRTs rely on distributional assumptions or a large number of clusters to maintain nominal ...
Rabideau, Dustin J, Wang, Rui
core   +2 more sources

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

The prevention and reduction of weight loss in an acute tertiary care setting: Protocol for a pragmatic stepped wedge randomised cluster trial (the PRoWL Project) [PDF]

, 2013
Background: Malnutrition, with accompanying weight loss, is an unnecessary risk in hospitalised persons and often remains poorly recognised and managed.
A Dickinson, A Jaafar, A Kitson, A Kitson, A Kitson, A Westergren, AC Milne, Age UK, AL Kitson, Alison L Kitson, Alison Shanks, AM Mudge, AM Mudge, Australian Council on Healthcare Standards, C Brown, C Davis, C Liddy, C Mouchoux, C Watterson, C Watterson, C Xia, CA Brown, CA Estabrooks, CA Lamb, CA Russell, D Jefferies, D Moher, D Vanderkroft, DG Duncan, DH Sullivan, DH Sullivan, E Parsons, F Neelemaat, GS Doig, HM Kruizenga, I Boutron, I Boutron, Ian Chapman, J Crosbie, J Kondrup, J Larsson, J Porter, JC Hoekstra, JP McWhirter, K Walton, KE Covinsky, L Bradley, L Brugler, L Villalon, L Wright, Leslye Long, LJ Ross, M Elia, M Elia, M Elia, M Hickson, M Zwarenstein, MA Hussey, MF Tsang, MH Middleton, MK Campbell, N Kagansky, N Laird, National Institute for Health and Clinical Excellence, ND Mdege, R Bailey, R Pawson, R Raja, R Thibault, R Wiechula, Rick Wiechula, RJ Stratton, RJ Stratton, RJ Stratton, RJ Stratton, S Holmes, S Klein, S Naithani, S Wong, SM Green, SM Green, SM Schneider, Stijn Soenen, TF Amaral, Timothy J Schultz, TJ Schultz   +85 more
core   +3 more sources

A Step-Wedge in the Biochemical Diagnosis of Preeclampsia [PDF]

Clinical Chemistry, 2019
Preeclampsia, a hypertensive disorder of pregnancy, is one of the leading causes of maternal and neonatal morbidity and mortality worldwide. Among the reasons for the great global burden of disease is the poor predictive value of high blood pressure and proteinuria (traditional markers for the disease) for related adverse outcomes (1).
Elizabeth R, Lemoine, S Ananth, Karumanchi   +1 more
openaire   +2 more sources

Aggressive prostate cancer is associated with pericyte dysfunction

Molecular Oncology, EarlyView.
Tumor‐produced TGF‐β drives pericyte dysfunction in prostate cancer. This dysfunction is characterized by downregulation of some canonical pericyte markers (i.e., DES, CSPG4, and ACTA2) while maintaining the expression of others (i.e., PDGFRB, NOTCH3, and RGS5).
Anabel Martinez‐Romero, Ane Martinez‐Larrinaga, Joaquim Grego‐Bessa, Saioa Garcia‐Longarte, Hielke van Splunder, Ianire Astobiza, Amaia Ercilla, Laura Bozal‐Basterra, Isabel Mendizabal, Pilar Villacampa, Arkaitz Carracedo, Mariona Graupera   +11 more
wiley   +1 more source

Rollout trial designs in implementation research are often necessary and sometimes preferred

Implementation Science
Background Rollout designs, which include stepped wedge designs, are defined by staggered implementation of new or alternative programs or services. Critiques of stepped wedge and other rollout designs have raised concerns regarding the confounding of ...
Gregory E. Simon, Bryan R. Garner, Justin D. Smith, Peter A. Wyman, Theresa E. Matson, Lia Chin-Purcell, Ian Cero, Wouter Vermeer, Kimberly A. Johnson, Guillermo Prado, C. Hendricks Brown   +10 more
doaj   +1 more source

Sample size and power analysis for stepped wedge cluster randomised trials with binary outcomes

Statistical Theory and Related Fields, 2021
In stepped wedge cluster randomised trials (SW-CRTs), clusters of subjects are randomly assigned to sequences, where they receive a specific order of treatments.
Jijia Wang, Jing Cao, Song Zhang, Chul Ahn   +3 more
doaj   +1 more source

Methylation biomarkers can distinguish pleural mesothelioma from healthy pleura and other pleural pathologies

Molecular Oncology, EarlyView.
We developed and validated a DNA methylation–based biomarker panel to distinguish pleural mesothelioma from other pleural conditions. Using the IMPRESS technology, we translated this panel into a clinically applicable assay. The resulting two classifier models demonstrated excellent performance, achieving high AUC values and strong diagnostic accuracy.
Janah Vandenhoeck, Nele De Meulenaere, Thomas Vanpoucke, Joe Ibrahim, Dieter Peeters, Suresh Krishan Yogeswaran, Wen Wen, Paul Van Schil, Jeroen M. H. Hendriks, Jo Raskin, Jan van Meerbeeck, Guy Van Camp, Ken Op de Beeck   +12 more
wiley   +1 more source

Designing stepped wedge trials to evaluate physical activity interventions in schools: methodological considerations

International Journal of Behavioral Nutrition and Physical Activity
Background In a stepped wedge design, schools are randomised to a sequence of measurements, with each sequence transitioning to intervention status at a different time.
Ruth Salway, Danielle House, Simona Kent-Saisch, Robert Walker, Lydia Emm-Collison, Alice Porter, David R Lubans, Michael Beets, Frank de Vocht, Russell Jago   +9 more
doaj   +1 more source