Results 41 to 50 of about 6,729 (263)

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Pragmatic cluster randomized trial to evaluate effectiveness and implementation of EHR-facilitated collaborative symptom control in cancer (E2C2): addendum

Trials, 2023
We previously described the hypotheses, outcomes, design, and analysis for E2C2, a pragmatic stepped-wedge trial to assess an intervention to improve symptom control in patients with cancer.
Jeph Herrin, Lila J. Finney Rutten, Kathryn J. Ruddy, Kurt Kroenke, Andrea L. Cheville   +4 more
doaj   +1 more source

PrEP uptake and HIV viral suppression when PrEP is integrated into Ugandan ART clinics for HIV-negative members of HIV-serodifferent couples: A stepped wedge cluster randomized trial

EClinicalMedicine, 2022
Summary: Background: Global scale-up of HIV pre-exposure prophylaxis (PrEP) includes services to HIV-negative people in partnerships with people living with HIV (serodifferent couples).
Renee Heffron, Timothy R. Muwonge, Katherine K. Thomas, Florence Nambi, Lylianne Nakabugo, Joseph Kibuuka, Dorothy Thomas, Erika Feutz, Allison Meisner, Norma C. Ware, Monique A. Wyatt, Jane M. Simoni, Ingrid T. Katz, Herbert Kadama, Jared M. Baeten, Andrew Mujugira, Renee Heffron, (protocol chair), Jared M. Baeten, Jane Simoni, Deborah Donnell, Ruanne Barnabas, Katherine K. Thomas, Dorothy Thomas, Erika Feutz, Cole Grabow, Allison Meisner, Kristin Ciccarelli, Caitlin Scoville, Katrina Ortblad, Andrew Mujugira, Timothy R. Muwonge, Joseph Kibuuka, Lylianne Nakabugo, Florence Nambi, Mai Nakitende, Diego Izizinga, Vicent Kasita, Brenda Kamusiime, Alisaati Nalumansi, Collins Twesige, Grace Kakoola, Charles Brown, Sylvia Namanda, Herbert Kadama, Norma C. Ware, Monique A. Wyatt, Emily Pisarski, Ingrid T. Katz   +47 more
doaj   +1 more source

Health Economic Evaluation Alongside Stepped Wedge Trials: A Methodological Systematic Review [PDF]

PharmacoEconomics, 2020
Recently, there has been an increase in use of the stepped wedge trial (SWT) design in the context of health services research, due to its pragmatic and methodological advantages over the parallel group design.Our objective was to summarise the statistical methods used when conducting economic evaluations alongside SWTs.A systematic literature search ...
Thomas Lung, Lei Si, Richard Hooper, Gian Luca Di Tanna   +3 more
openaire   +3 more sources

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

RACE-IT – Rapid Acute Coronary Syndrome Exclusion using the Beckman Coulter Access high-sensitivity cardiac troponin I: A stepped-wedge cluster randomized trial

Contemporary Clinical Trials Communications, 2021
Background: Protocols utilizing high-sensitivity cardiac troponin (hs-cTn) assays for the evaluation of suspected acute coronary syndrome (ACS) in the emergency department (ED) have been gaining popularity across the US and the world.
Joseph Miller, Bernard Cook, Gulmohar Singh-Kucukarslan, Amy Tang, Shooshan Danagoulian, Gerard Heath, Ziad Khalifa, Phillip Levy, Simon A. Mahler, Nicholas Mills, James McCord   +10 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Vaccine trials during a pandemic: potential approaches to ethical dilemmas

Trials, 2021
Ever since the emergence of the coronavirus disease 2019 (COVID-19), global public health infrastructures and systems, along with community-wide collaboration and service, have risen to an unprecedented challenge.
Manaf Alqahtani, Saad I. Mallah, Nigel Stevenson, Sally Doherty   +3 more
doaj   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Rollout trial designs in implementation research are often necessary and sometimes preferred

Implementation Science
Background Rollout designs, which include stepped wedge designs, are defined by staggered implementation of new or alternative programs or services. Critiques of stepped wedge and other rollout designs have raised concerns regarding the confounding of ...
Gregory E. Simon, Bryan R. Garner, Justin D. Smith, Peter A. Wyman, Theresa E. Matson, Lia Chin-Purcell, Ian Cero, Wouter Vermeer, Kimberly A. Johnson, Guillermo Prado, C. Hendricks Brown   +10 more
doaj   +1 more source