Results 221 to 230 of about 8,381,892 (293)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Quality of life before and after catheter ablation (pulmonary vein isolation) for atrial fibrillation: Results from the Netherlands Heart Registration. [PDF]

Neth Heart J
Oirbans T, de Jong JSSG, van Steenbergen GJ, Adiyaman A, Schoonderwoerd BA, Rijnhart-de Jong HG, van der Voort PH, Luermans JGLM, Westra SW, Kuijt WJ, van der Stoel MD, Kelder JC, Boersma LVA, Balt JC.   +13 more
europepmc   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Navigating new norms: a systematic review of factors for the development of effective digital tools in higher education

FEBS Open Bio, EarlyView.
What factors make for an effective digital learning tool in Higher Education? This systematic review identifies elements of a digital tool that published examples reveal to be features of an engaging and impactful digital tool. A systematic literature search yielded 25 research papers for analysis.
Akmal Arzeman, Jessica Haines, Connie Pritchard, Stephen Rutherford, Nigel Francis   +4 more
wiley   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

Between Hearts and Gears: Technology at the Service of Life. [PDF]

Braz J Cardiovasc Surg
Duarte Junior PC, Murakami AN, Andrade AJP.   +2 more
europepmc   +1 more source

Pathologic complete response in rectal cancer: advocating for local excision. [PDF]

Ann Coloproctol
Gonzalez-Nunez D, Torres L, Gonzalez-Dorado M, Reyes JC.   +3 more
europepmc   +1 more source

New perspectives in the management of adults with congenital heart disease: insights from the 2025 ACHD Congress. [PDF]

Eur Heart J Suppl
Borrelli N, Sabatino J, Ruperti-Repilado FJ, Tutarel O, Giannakoulas G, D'Alto M, Romeo E, Tobler D, Diller GP.   +8 more
europepmc   +1 more source

Sources of information on HIV/AIDS used by adolescents and young people: A scoping review protocol. [PDF]

PLoS One
do Nascimento Gomes Brito VC, de Souza Maximiano LC, de Andrade FB, de Abrantes RM, Gañan Rojas YL, de Medeiros JA, de Andrade RM, de Freitas Costa EN, da Silva Guedes S, Felipe BOF, Feijão AR.   +10 more
europepmc   +1 more source