Results 201 to 210 of about 634,953 (304)

Transient Transfection of Rolling‐Circle Amplified DNA in Biomanufacturing‐Relevant Mammalian Cell Lines: A Comparison of Transfection Conditions for Optimal Protein Expression

open access: yesBiotechnology and Applied Biochemistry, EarlyView.
ABSTRACT Rolling‐circle amplification (RCA) is a completely synthetic and rapid mechanism for scaling‐up bulk DNA that eliminates requirements for large‐scale bacterial fermentation. Several reports have described the surprising ability of large unprocessed (hyperbranched) rolling circle‐amplified DNA (RCA DNA) to effectively transfect cultured cells ...
Evelina Loghin   +8 more
wiley   +1 more source

Optimizing biomass use for carbon storage and land use with the OptiBiU model

open access: yesBiofuels, Bioproducts and Biorefining, EarlyView.
Abstract Optimal biomass use in a circular bioeconomy requires a system perspective to guide decision‐making. The Optimizing Biomass Use (OptiBiU) model was developed for this purpose. The model distinguishes among primary biomass production, intermediate products, and biobased products, and incorporates recycling.
Pim M. Post   +8 more
wiley   +1 more source

Design of experiments‐driven optimization of Porphyridium marinum cultivation: impact of operational variables on phycoerythrin and exopolysaccharide production

open access: yesBiofuels, Bioproducts and Biorefining, EarlyView.
Abstract Among the Porphyridium genus, Porphyridium marinum exhibits the highest phycoerythrin (PE) content. In this study, the metabolic trade‐off between biomass, PE, and sulfated exopolysaccharide (EPS) production was assessed under varying nitrogen and sulfur availability, light intensity, residence time, and cultivation mode.
Rosaria Tizzani   +3 more
wiley   +1 more source

Life cycle assessment of biodiesel synthesis from waste cooking oil using bifunctional heterogeneous catalyst

open access: yesBiofuels, Bioproducts and Biorefining, EarlyView.
Abstract The development of renewable energy sources is important in order to achieve the United Nations Sustainable Development Goals (SDGs). Waste cooking oil (WCO) is a promising feedstock for biodiesel production through transesterification. Heterogeneous bifunctional catalysts offer a sustainable alternative to conventional homogeneous bases such ...
Frederick Jit Fook Phang   +9 more
wiley   +1 more source

Metabolic fate of drugs of abuse and new psychoactive substances: A pilot study on a novel workflow using a zebrafish embryo model combined with human microdosing

open access: yesBritish Journal of Clinical Pharmacology, EarlyView.
Aim The aim of this study was to develop a novel workflow to identify human urine biomarkers for drugs of abuse and new psychoactive substances. Metabolites of amphetamine, cocaine, LSD, MDMA, methamphetamine, THC, MDMB‐CHMICA, and MDPPP were first identified in a zebrafish embryo (ZE) metabolism study followed by comparison to most abundant human ...
Simon K. Wellenberg   +7 more
wiley   +1 more source

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